Assessment of serological tests for the diagnosis of canine visceral leishmaniasis |
| |
| Authors: | Denise Amaro da Silva Maria de Fátima Madeira Tuanne Rotti Abrantes Carlos José de Lima Barbosa Filho Fabiano Borges Figueiredo |
| |
| Affiliation: | 1. Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;2. Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;3. Centro de Pesquisas Rene Rachou/FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil;4. Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil;5. Faculdade de Medicina, Universidade Federal de Goiás, Instituto Goiano de Oncologia e Hematologia (INGOH), Goiânia, Goiás, Brazil;6. Instituto Evandro Chagas MS/SVS/Brasil, Departamento de Parasitologia, Laboratório de Leishmanioses Prof. Dr. Ralph Lainson, Ananindeua, Pará, Brazil;7. Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Minas Gerais, Brazil;1. Dermatomycology Laboratory, School of Medicine, Pós-graduação em Ciências Médicas, University of Brasília (Universidade de Brasília), Brasília, Brazil;2. Laboratory of Molecular Biology, Division of Dermatology, Clinical Hospital of Ribeirão Preto, Sao Paulo, Brazil;3. Dermatology Department, University of Brasília (Universidade de Brasília), Brasília, Brazil;4. Laboratory of Molecular Biology, Division of Dermatology, Department of Medical Clinics, School of Medicine of Ribeirao Preto, University of São Paulo, Sao Paulo, Brazil;5. Dermatology Department, Department of Clinical Medicine, University of Brasília (Universidade de Brasília), Brasília, Brazil |
| |
| Abstract: | An immunoenzymatic assay (ELISA), an indirect immunofluorescence antibody test (IFAT) with different antigens (ELISA-Leishmania chagasi, ELISA-L. major-like, IFAT-L. chagasi and IFAT-L. major-like), and an immunochromatographic test were assessed for the diagnosis of canine visceral leishmaniasis (CVL). Serum samples from 144 dogs from an endemic area for visceral leishmaniasis in the municipality of Rio de Janeiro were tested. The sensitivities of the serological tests were 93%, 100%, 73%, 60% and 93%, with specificities of 87%, 92%, 77%, 96% and 92% for the ELISA-L. major-like, ELISA-L. chagasi, IFAT-L. major-like, IFAT-L. chagasi and the immuno chromatographic test, respectively. ELISA-L. chagasi was the best test for the diagnosis of CVL, but the immunochromatographic test could be a useful alternative as it offers simple and rapid diagnosis without the need for a specialized laboratory. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
