| 植酸酶产酶条件及热稳定性的研究 |
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| 引用本文： | 陈惠,王红宁.植酸酶产酶条件及热稳定性的研究[J].四川农业大学学报,1999,17(3):291-294. |
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| 作者姓名： | 陈惠 王红宁 |
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| 作者单位： | 1. 四川农业大学,基础部
2. 四川农业大学,动物科技学院,四川,雅安,625014 |
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| 基金项目： | 国家“九五”科技攻关计划资助 |
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| 摘 要： | 对黑曲霉９７０１、米曲霉９７１２ 、根霉９７０２ 三种霉菌进行固体培养法生产植酸酶的条件研究。结果表明：在３０ ℃±１℃条件下，黑曲霉９７０１ 培养７ｄ 产酶活力达１４０５２ｐｕ／ｇ；米曲霉９７１２ 培养６ｄ，产酶活力达１５３８７ｐｕ／ｇ；根霉９７０２ 培养６ｄ，产酶活力达１４０７０ｐｕ／ｇ。对黑曲霉９７０１ 用缓冲液（ｐＨ４８） 控制ｐＨ，在３０ ℃±１℃培养７ｄ，产酶水平达２１３１０ｐｕ／ｇ 。将黑曲霉９７０１ 植酸酶液在９０ ℃条件下保温１５ｍｉｎ，仍保留７６ ％ 的原酶活性。
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| 关 键 词： | 植酸酶活性 黑曲霉 米曲霉 根霉 热稳定性 |
| 文章编号： | 1000-2650(1999)03-0291-04 |
| 修稿时间： | 1999-07-19 |
Studies on Condition of Producing Phytase and Thermostability Properties |
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| CHEN Hui,WANG Hong-ning.Studies on Condition of Producing Phytase and Thermostability Properties[J].Journal of Sichuan Agricultural University,1999,17(3):291-294. |
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| Authors: | CHEN Hui WANG Hong-ning |
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| Abstract: | The condition of producing phytase of Asp.niger9701, Asp.oryzae9712 and rhizopus sp. 9702 are compared in solid state fermentation process. The results show: Asp.niger 9701 is incubated at 30℃±1℃ for 7 days, and it can produce 14052pu/g; Asp.oryzae 9712 and rhizopus sp. 9702 are incubated at 30℃±1℃ for 6 days, and they can produce 15387pu/g and 14070pu/g, respectively. In addtion, by controlling the pH of the buffer Asp.niger 9701 can produce phytase to 21310pu/g. When the phytase of Asp. Niger 9701 is put into 90℃ for 15min, the activity retains by 76%. |
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| Keywords: | PHYTASE ACTIVITY ASP NIGER ASP ORYZAE RHIZOPUS SP THERMOSTABILITY |
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