巴什拜羊与其杂交羊对绵羊肺炎支原体感染的比较

为了比较巴什拜羊与其杂交羊对绵羊肺炎支原体(MO)感染的差异,将6只巴什拜羊和6只杂交羊人工感染MO,分别观察其临床症状和病理解剖变化,用ELISA方法分别检测血清中IL-1β、IL-6、IL-9及IFN-γ的浓度,制作肺部组织切片观察组织病理学变化,并进行组织病理学评分。结果显示,感染后杂交羊比巴什拜羊表现出更严重的、典型的支原体肺炎的临床症状和病理剖检变化。组织病理学评分结果显示,杂交羊的评分显著高于巴什拜羊。相关细胞因子检测结果显示,杂交羊血清中IL-1β、IL-6、IL-9及IFN-γ的浓度均显著高于巴什拜羊。结果表明,巴氏拜羊对MO感染具有一定的抗性,而杂交羊对MO较易感。

Comparison of Mycoplasma ovipneumoniae Infection between Bashbay Sheep and Its Crossbred Sheep

In order to compare the difference of Mycoplasma ovipneumoniae(MO) infection between Bashbay sheep and its crossbred sheep,6 Bashbay sheep and 6 hybrid sheep were infected with MO,and their clinical symptoms and pathological lesions were observed.IL-1β,IL-6,IL-9 and IFN-γ concentrations in sera were measured by ELISA.Histopathological lesions of lung tissues were observed by pathological sections and histopathological scores were obtained.The results showed that the post-infection hybrid sheep represented more severe,typical mycoplasma pneumonia clinical symptoms and pathological lesions than Bashbay sheep.The results of histopathological scoring found that the hybrid sheep was significantly higher than Bashbay sheep.The results of cytokine test showed that the concentrations of IL-1β,IL-6,IL-9 and IFN-γ in sera of hybrid sheep were significantly higher than those of Bashbay sheep.Bashbay sheep has certain resistance to MO infection,while hybrid sheep was more susceptible to MO.