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| 辣椒根腐病拮抗细菌的筛选、鉴定及其抑菌促生作用 | |
| 引用本文： | 曲春鹤,何付丽,刘培福,纪明山,赵长山,高黎力. 辣椒根腐病拮抗细菌的筛选、鉴定及其抑菌促生作用[J]. 东北农业大学学报, 2012, 0(7): 89-94 |
| 作者姓名： | 曲春鹤 何付丽 刘培福 纪明山 赵长山 高黎力 |
| 作者单位： | 1. 东北农业大学农学院,哈尔滨150030 2. 沈阳农业大学植物保护学院,沈阳110161 3. 黑龙江省农药管理检定站哈尔滨150090 |
| 基金项目： | 国家“十一五”科技支撑计划课题(2006BAD07B02) |
| 摘 要： | 采用平板对峙-胚根试验-盆栽试验系统筛选方法,以Fusarium solani为指示菌,从黑龙江省辣椒根际土壤中筛选得到4株拮抗细菌,分别命名为菌株 D221、II621、15-1-1、19-2-3.菌株 D221对大豆疫病菌(Phytophthora sojae)、大豆菌核病菌(Sclerotinia sclerotinorum)、黄瓜枯萎病菌(F. oxysporum)、黄瓜立枯病菌(Rhizoctonia solani)、小麦根腐病菌(Bipolaris sorokiniana)、棉花黄萎病菌(Verticillium dahliae)、水稻恶苗病菌(F. moniliforme)具有明显拮抗作用,对辣椒胚根生长具有促进作用.经形态学、生理生化鉴定及16S rDNA分析,将菌株D221鉴定为枯草芽胞杆菌(Bacillus subtilis),并在GenBank中注册,序列号为JF495161 |
| 关 键 词： | 辣椒根腐病 拮抗细菌 筛选 鉴定 抑菌效果 |
Selection and identification of antagonistic bacteria against Fusarium solani and its effect |
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| QU Chunhe,HE Fuli,LIU Peifu,JI Mingshan,ZHAO Changshan,GAO Lili. Selection and identification of antagonistic bacteria against Fusarium solani and its effect[J]. Journal of Northeast Agricultural University, 2012, 0(7): 89-94 | |
| Authors: | QU Chunhe HE Fuli LIU Peifu JI Mingshan ZHAO Changshan GAO Lili |
| Affiliation: | 1.School of Agriculture,Northeast Agricultural University,Harbin 150030,China;2.School of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China;3.Heilongjiang Pesticide Management and Dectection Station,Harbin 150090,China) |
| Abstract: | With Fusarium solani as indicator,four antagonistic bacteria were selected from the rhizosphere of Capsicum annuum in Heilongjiang Province,and named D221,II621,15-1-1,and 19-2-3,respectively.Strain D221 was evaluted in culture assay,and several different pathogens including Phytophthora sojae,Sclerotinia sclerotinorum,F.oxysporum,Rhizoctonia solani,Bipolaris sorokiniana,Verticillium dahliae and F.moniliforme were inhibited,significantly.Pepper radicle growth was promoted.The strain D221 was identified as Bacillus subtilis by morphological test,physiological and biochemical tests and 16S rDNA sequence analysis.And the sequence was registered in GenBank getting the accession No.JF495161. |
| Keywords: | Fusarium solani antagonistic bacterium selection identification control effect |
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