| 江西省主要外来猪种氟烷基因分布频率的初步研究 |
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| 引用本文： | 丁能水,黄路生.江西省主要外来猪种氟烷基因分布频率的初步研究[J].江西农业大学学报,1999,21(4):555-557. |
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| 作者姓名： | 丁能水 黄路生 |
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| 作者单位： | 江西农业大学动物科学技术学院 |
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| 基金项目： | 江西省跨世纪学术和技术带头人培养计划资助 |
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| 摘 要： | 从耳组织提取基因组ＤＮＡ，利用ＰＣＲ－ＲＦＬＰ技术检测了江西省三个主要外来猪种杜洛鸹９７头、长白１０２头、大约克１４０头共３３９头种猪的氟烷基因型。结果表明：在所有三个品种中均没有检测到隐性纯合子ｎｎ；显性纯合子ＮＮ和杂和子Ｎｎ在上述三个品种中的基因型频率分别为９５．８８％，９７．０６％，９１．４３％，４．１２％，２．９４％，８．５７％；等位基因ｎ的基因频率在上述三个品种分别为２．０６％，１．４７
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| 关 键 词： | 氟烷基因 PCR－RFLP 基因频率 猪 |
The Preliminary Study on Distributive Frequencies of Halothane Gene in Three Major Introduced Pig Breeds in Jiangxi Province |
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| Ding Nengshui,Huang Lusheng,Ren Jun,Gao Jun,Chen Kefei,Lin Wanhua,Zhou Lihua,Li Lin.The Preliminary Study on Distributive Frequencies of Halothane Gene in Three Major Introduced Pig Breeds in Jiangxi Province[J].Acta Agriculturae Universitis Jiangxiensis,1999,21(4):555-557. |
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| Authors: | Ding Nengshui Huang Lusheng Ren Jun Gao Jun Chen Kefei Lin Wanhua Zhou Lihua Li Lin |
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| Abstract: | DNA was extracted from ear tissue and the genotypes for halothane were detected in three major introduced pig breeds in Jiangxi Province including 97 Durocs, 102 Landraces and 128 Large Yorkshires using PCR-RFLPs. The results showed as follows: no homozygous recessive genotype nn was detected. The genotype frequencies of homozygous NN and heterozygous Nn in the three examined breeds were 95.88%,97.06%,93.75%and 4.12% ,2.94%,6.25% respectively, while the gene frequencies of allele n in the three breeds were 2.06%, 1.47% and 3.13%respectively, which were significantly lower than those in other similar reports in various countries or districts. The reason for the difference of halothane gene distributive frequencies between breeds and regions together with relevant breeding strategy were also discussed. |
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| Keywords: | halothane gene PCR-RFLP frequency of gene |
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