鹌鹑源火鸡隐孢子虫在小白鼠和雏鸡体内内生发育阶段的超微结构比较

用鹌鹑源火鸡隐孢子虫（Cryptos poridium meleagridis）卵囊分别感染昆明系小白鼠和固始雏鸡，用透射电镜和扫描电镜观察比较了C．meleagridis在两种试验动物体内的内生发育虫体超微结构和致病性的差异。利用扫描电镜观察发现，鹌鹑源火鸡隐孢子虫（C.meleagridis）在小白鼠和雏鸡体内的寄生部位有较大差异，在小白鼠体内寄生于十二指肠，但在雏鸡体内主要寄生于回肠；C．meleagridis深嵌于小白鼠肠微绒毛丛内，微绒毛较为完整；但在雏鸡回肠，C．meleagridis似黏附在肠黏膜表面，微绒毛脱落明显，对雏鸡致病性明显比对小白鼠的致病性强。利用透射电镜在两种试验动物的样品中均观察到不同发育阶段的滋养体、裂殖体和大配子体以及正在孢子化的卵囊。滋养体和裂殖体在发育过程中可明显见到粗面内质网结构，裂殖生殖中期阶段粗面内质网尤其发达；小白鼠体内的C．meleagridis虫体与肠黏膜接触处形成一凹陷，寄生部位较深，而在雏鸡体内无此现象。此外，利用透射和扫描电镜均观察到虫体寄生部位周围微绒毛密度高而且也比其它部位长。形成这些差异的原因有待于进一步探讨。

Comparison of Ultrastructure in Endogenous Development Stages of Cryptosporidium meleagridis between Chickens and Mice

Oocysts of C.meleagridis from quail were orally inoculated into Kunming mice and Gushi chickens to study endogenous development and pathogenicity respectively.The comparison of ultrastructure in endogenous development stages of C.meleagridis between chickens and mice was studied by scanning and transmission electron microscope.The parasitic site of C.meleagridis in mice was different from that in chickens.Cryptosporidia was detected in duodenum of mice but in ileum of chickens by scanning electron microscope.The pathogenicity of the C.meleagridis to mice and chickens is different too.Cryptosporidia was embedded deeply in microvillus of duodenum of mice,and microvilli were damaged slightly by the parasite,while cryptosporidia seemed to attach to mucous membrane of ileum in chickens,and microvilli desquamated seriously.It was concluded that C.meleagridis caused more serious damage in chickens than that in mice.Trophozoite,schizont,macrogamont,and sporulating oocyst were observed in mice and chickens by transmission electron microscope respectively,and the rough endoplasmic reticula were rich in trophozoite and schizont,particularly in middle development stage of schizogony.The depressed niche that parasites resided can be observed in the boundary between cryptosporidia and intestinal mucosa in mice by transmission electron microscope,but can't be found in chickens.The denser and higher microvilli were discovered around the parasite than the others in mice by electron microscope.The reasons for the difference above between mice and chickens need further research.These should be new biologic characteristic of C.meleagridis.