| 富士苹果AFLP体系的优化及其在鉴定早熟芽变中的应用 |
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| 引用本文： | 李元军,唐美玲,于青,刘美英,宋来庆,韩振海.富士苹果AFLP体系的优化及其在鉴定早熟芽变中的应用[J].园艺学报,2009,36(3):327-332. |
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| 作者姓名： | 李元军 唐美玲 于青 刘美英 宋来庆 韩振海 |
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| 作者单位： | （烟台农业科学研究院果树所，山东烟台265500；中国农业大学园艺植物研究所，北京 100094） |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 以‘长富2号’苹果为材料，采用优化的AFLP技术对其早熟芽变与母株进行初步研究，筛选了64对引物，其中43对引物扩增结果理想，共产生了2,700条带。有4对引物在芽变与母株之间产生了7条差异片段，主要集中在350~800 bp之间。研究表明早熟芽变是由于遗传物质改变造成的，同时也说明利用该体系对苹果芽变进行分析和鉴定获得成功。
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| 关 键 词： | 苹果 AFLP 芽变 |
| 收稿时间： | 2008-10-17 |
Optimization of AFLP System for Fuji and Identification Early-maturing Sport from 'Changfu 2' |
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| LI Yuan-jun,TANG Mei-ling,YU Qing,LIU Mei-ying,SONG Lai-qing,HAN Zhen-hai.Optimization of AFLP System for Fuji and Identification Early-maturing Sport from 'Changfu 2'[J].Acta Horticulturae Sinica,2009,36(3):327-332. |
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| Authors: | LI Yuan-jun TANG Mei-ling YU Qing LIU Mei-ying SONG Lai-qing HAN Zhen-hai |
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| Institution: | (Fruit Institute of Yantai Agricultural Science Academy, Yantai, Shandong 265500,China; Institute of Horticultural Plants，China Agricultural University，Beijing 100094，China) |
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| Abstract: | Using AFLP to compare the early-maturing sport and its mother plant in ‘Changfu 2’．Sixty-four selective primer pairs was adopted in AFLP analysis. 2,700 clear bands was detected by 43 pairs of AFLP primers. There were 7 obvious bands difference between early-maturing sport and mother plant under 4 pair primers. The molecular weight range of the polymorphic fragments was mainly 350~800 bp．The results indicated that there were some molecular-level differentiation between 'Changfu 2' and its early-maturing sport. The application of AFLP in the research of apple bud mutation is Successfu1. |
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| Keywords: | AFLP |
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