用DNA差异显示技术分离、测序和鉴定猪的两个新分子标记及其与性状的关联分析

为了寻找更多的和性状关联的猪候选基因或分子标记,用DNA差异显示技术扫描140头大白×梅山F2猪的DNA,发现了2个与猪的性状具有显著关联的分子标记,并对这2个分子标记进行测序和鉴定.标记1与最后肋骨处估测背膘厚(P＜0.01)、倒数三四肋骨处估测背膘厚(P＜0.01)、估测瘦肉率(P＜0.01)、骨重(P＜0.05)、骨率(P＜0.05)、肥肉重(P＜0.05)、肥肉率(P＜0.05)、瘦肥比率(P＜0.05)、肩部最厚处背膘厚(P＜0.05)、6～7胸椎间背膘厚(P＜0.01)等性状关联.标记2与背最长肌pH值(P＜0.05)、失水率(P＜0.05),系水力(P＜0.05),背最长肌肉色评分(P＜0.05),背最长肌含水量(P＜0.05)、肋骨数(P＜0.05)骨率(P＜0.05)、胸腰椎结合处背膘厚(P＜0.05)、胃净重(P＜0.01)等性状关联.经与GenBank进行Blast比较,这2个标记与已知的猪基因或基因组序列没有明显的同源性,提交到GenBank,登陆号分别为CN605659和AY626262.研究所用的方法为揭示新的猪候选基因或分子标记提供了一条新的途径.

Isolation, Sequencing and Identification of Two Pig Novel Molecular Markers Using DNA Differential Display Technique and Their Trait Analysis

In order to find more candidate genes or DNA molecular markers which are correlated with the traits, we used DNA differential display technique to scan the whole genome of F2 pig population based on Large White xMeishan, and two molecular markers were found to be significantly correlated with the traits. Marker 1 was associated with: estimated backfat thickness at last rib (P＜ 0.01), estimated backfat thickness at last 3～4th rib(P＜ 0.01), estimated lean meat percentage (P＜ 0.01), bone weight (P ＜ 0.05),bone percentage (P ＜ 0.05), fat meat weight (P ＜ 0.05), fat meat percentage (P ＜ 0.05), ratio of lean meat to fat meat (P ＜ 0.05), backfat thickness at shoulder (P ＜ 0.05), backfat thickness at 6-7th thorax (P ＜ 0.01), and marker 2 was associated with: longissimus dorsi pH (P＜ 0.05), drip loss rate (P ＜ 0.05), water holding capacity (P ＜ 0.05), meat color score oflongissimus dorsi (P ＜ 0.05), water moisture of longissimus dorsi (P ＜ 0.05), rib numbers (P ＜ 0.05), bone percentage (P ＜ 0.05), backfat thickness at thorax-waist (P ＜ 0.05),gaster net weight (P ＜ 0.01). The two markers were then isolated, sequenced and identified. BLAST analysis revealed that the two markers were not homologous to any of the known porcine genes from GenBank and subsequenfiy submitted to GenBank database(Accession number: CN605659 and AY626262). The method used in this study provides an additional useful tool in the investigation of candidate genes or DNA molecular markers, which were correlated with the traits.