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甜瓜自交系不定芽再生过程中内源激素含量的变化
引用本文:张慧君,栾非时,王学征,高鹏,牛明明.甜瓜自交系不定芽再生过程中内源激素含量的变化[J].中国蔬菜,2012,1(24):43-47.
作者姓名:张慧君  栾非时  王学征  高鹏  牛明明
作者单位:东北农业大学园艺学院,黑龙江哈尔滨 150030
基金项目:国家自然科学基金项目(31071812);国家西甜瓜产业技术体系分子育种岗位专家项目(CARS-26-02)
摘    要:以甜瓜自交系子叶节为试材,对不同再生能力的甜瓜自交系不定芽再生过程中内源激素含量的变化进行了测定。结果表明:子叶节接种初期诱导细胞启动分化时ZR、IAA、ABA和GA3含量均较高,细胞旺盛分裂期及芽原基形成期容易再生品系ZR含量较高,而IAA含量较低;容易再生品系ZR/ABA的比值一直保持较高水平,而IAA/ZR的比值一直保持较低水平。表明甜瓜子叶节不定芽形成与其内源激素的含量和比值密切相关。

关 键 词:甜瓜  子叶节  不定芽  再生  内源激素  

Changes of Endogenous Hormone Contents during in vitro Regeneration of Adventitious Bud of Cucumis melo L.Inbred Line
ZHANG Hui-jun , LUAN Fei-shi , WANG Xue-zheng , GAO Peng , NIU Ming-ming.Changes of Endogenous Hormone Contents during in vitro Regeneration of Adventitious Bud of Cucumis melo L.Inbred Line[J].China Vegetables,2012,1(24):43-47.
Authors:ZHANG Hui-jun  LUAN Fei-shi  WANG Xue-zheng  GAO Peng  NIU Ming-ming
Institution:Horticulture College,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
Abstract:Taking cotyledonary nodes collected from melon(Cucumis melo L.)as experimental materials,this study tested the changes of hormones contents during in vitro regeneration of adventitious buds.The results indicated that the contents of ZR,IAA,ABA and GA3 culture were higher during the early stage of inoculation,and the ZR contents were higher at the stage of cell vigorous division and bud primordium formation,while the IAA contents were lower.Compared with lines with different regeneration capacity,the level of ZR/ABA of easy regeneration line was higher,while the IAA/ZR kept at a lower level,indicating adventitious bud formation from cotyledonary node of melon was closely associated with the contents of endogenous hormone.
Keywords:Cotyledonary nodes  Adventitious bud  Regeneration  Endogenous hormone
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