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菜豆(油豆角)炭疽病菌的分离鉴定及抗病资源筛选
引用本文:张婷,胡国富,肖子恒,李鹤春,詹云.菜豆(油豆角)炭疽病菌的分离鉴定及抗病资源筛选[J].中国蔬菜,2012,1(22):75-80.
作者姓名:张婷  胡国富  肖子恒  李鹤春  詹云
作者单位:1.东北农业大学生命科学学院,黑龙江哈尔滨 150030;2黑龙江省农业科学院园艺分院,黑龙江哈尔滨 150069
基金项目:黑龙江省博士后启动基金项目(LRB07-37);东北农业大学211人才专项
摘    要:对侵染菜豆(油豆角)品种群的炭疽病菌进行分离鉴定,并对该品种群进行抗炭疽病资源筛选。结果表明,通过形态学和分子鉴定,确定从油豆角炭疽病样本中分离纯化的病原菌为菜豆炭疽菌。利用该菌液分别侵染48份油豆角品种的苗期叶片和成熟期嫩荚,发现苗期抗病品种为44份,感病品种为4份,其中1份为高感品种;成熟期抗病品种为40份,感病品种为8份,其中高感品种为3份。以叶片和荚的病情指数为变量显示苗期和成熟期的抗性呈极显著相关,其相关系数分别为Kendall:0.315,Spearman:0.411。

关 键 词:菜豆(油豆角)  炭疽病  抗病资源  

Isolation and Identification of Colletotrichum and Screening of Anthracnose Resistant Varieties from Snap Bean
ZHANG Ting , HU Guo-fu , XIAO Zi-heng , LI He-chun , ZHAN Yun.Isolation and Identification of Colletotrichum and Screening of Anthracnose Resistant Varieties from Snap Bean[J].China Vegetables,2012,1(22):75-80.
Authors:ZHANG Ting  HU Guo-fu  XIAO Zi-heng  LI He-chun  ZHAN Yun
Institution:1.Life Science College of Northeast Agricultural University,Harbin 150030,Heilongjiang,China;2Horticulture Sub-academy,Heilongjiang Academy of Agricultural Sciences,Harbin 150069,Heilongjiang,China
Abstract:In this study,we isolate and identify Colletotrichum from snap bean(Phaseolus vulgaris L.),and screen anthracnose resistant resources from these varieties.The results show that the pathogenic bacterium separated by morphology and molecular identification is Colletotrichum. Leaves at seedling stage and pods at matured stage of 48 bean varieties were soaked in liquid of Colletotrichum.We found that in seedling stage 44 varieties were resistant,4 varieties were susceptible,and among which 1 variety was highly susceptible.In matured period,40 varieties were resistant,and 8 varieties were susceptible,and among which 3 varieties were highly susceptible.Correlation analysis was made by taking disease severity index of leaves and pods through SPSS software as varable force.The result shows resistances at seedling stage and maturing stage have very significant correlation.The related coefficients are Kendall0.315,Spearman0.411.
Keywords:Anthracnose  Disease resistant varieties
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