| 不同方法提取高致病性禽流感及新城疫病毒RNA效果的比较分析 |
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| 引用本文： | 吴蔚,詹文辉,李鹤,应清香,廖媛,王邦彦.不同方法提取高致病性禽流感及新城疫病毒RNA效果的比较分析[J].福建畜牧兽医,2014(6):7-8. |
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| 作者姓名： | 吴蔚 詹文辉 李鹤 应清香 廖媛 王邦彦 |
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| 作者单位： | 福建省南平市动物疫病预防控制中心 353000 |
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| 摘 要： | 高致病性禽流感以及新城疫对禽养殖户危害巨大，应用荧光RT-PCR方法对高致病性禽流感、新城疫病毒进行检测在病原学检测中占据优势。而荧光RT-PCR检测的核心是核酸物质的提取。本文将离心柱内硅基质膜提取法（柱膜法）与磁珠法作比较，探讨更适合基层兽医实验室的核酸提取方式。
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| 关 键 词： | 柱膜法 磁珠法 提取 比较 |
Comparison of different RNA isolation methods for Highly pathogenic avian influenza and Newcastle disease |
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| Wu Wei,Zhan Wenhui,Li He Ying,Qingxiang
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Liao Yuan,Wang Bangyan.Comparison of different RNA isolation methods for Highly pathogenic avian influenza and Newcastle disease[J].Fujian Journal of Animal Husbandry and Veterinary,2014(6):7-8. |
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| Authors: | Wu Wei Zhan Wenhui Li He Ying Qingxiang Liao Yuan Wang Bangyan |
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| Institution: | Wu Wei Zhan Wenhui Li He Ying Qingxiang Liao Yuan Wang Bangyan (Nanping Center for Animal Disease Control and Prevention, Fujian 353000) |
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| Abstract: | Highly pathogenic avian influenza and Newcastle disease do the great harm to poultry farmers, FQ-PCR have an advantage in detecting method of etiology and Nucleic acid extraction is the key. This article compares Centrifugal column extraction with mag-netic bead method in order to find a better way in Basic veterinary laboratory. |
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| Keywords: | Centrifugal column extraction magnetic bead method Extraction Comparison |
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