Molecular typing of rose cultivars using RAPDs

Summary

A rapid molecular typing method is described in this work. RAPD amplification products are very dependent upon various factors such as source of taq DNA polymerase, thermocycling programmes and DNA concentrations. This issue has been consequently been examined to establish a suitable experimental protocol. Subsequently, genomic DNA from 25 cultivars of rose were amplified using RAPD techniques with twenty 10-mer primers (Operon Kit A). The data obtained reveal no variability within cultivars and a high degree of variation between cultivars. With the patterns obtained with two of the primers (OPA-11 and OPA-17) all the rose cultivars were unequivocally identified. The results suggest that RAPD profiles provide a simple and efficient way to identify rose cultivars.