| 牛病毒性腹泻病毒RT-PCR诊断方法的建立 |
| |
| 引用本文： | 魏澍,刘金玲. 牛病毒性腹泻病毒RT-PCR诊断方法的建立[J]. 现代畜牧兽医, 2012, 0(3): 58-61 |
| |
| 作者姓名： | 魏澍 刘金玲 |
| |
| 作者单位： | 1. 辽宁省动物疫病预防控制中心,辽宁沈阳,110164
2. 沈阳农业大学畜牧兽医学院,辽宁沈阳,110866 |
| |
| 摘 要： | 根据已发表的牛病毒性腹泻病毒株的基因序列,分析合成了一对扩增跨幅为190bp左右的引物,对牛病毒性腹泻病基因I型或基因II型的毒株进行RT-PCR扩增,结果是取得了与预期大小一致的RT-PCR产物,而对照样品的扩增全为阴性;该方法最低可检测到0.10ng的牛病毒性腹泻病毒RNA。
|
| 关 键 词： | 牛病毒性腹泻病毒 RT-PCR诊断 |
The Foundation of PCR Diagnosis Method For Bovine Viral Diarrhea Virus |
| |
| Wei Shu,Liu jinling. The Foundation of PCR Diagnosis Method For Bovine Viral Diarrhea Virus[J]. Modern JOurnal of Animal Husbandry and Veterinary Medicine, 2012, 0(3): 58-61 |
| |
| Authors: | Wei Shu Liu jinling |
| |
| Affiliation: | 1.The Preventive Center of Animal Disease of LiaoNing Province, Liaoning Shenyang 110161; 2.The Institute of Husbandry and Veterinary Medicine of Shenyang Agriculture University, Liaoning Shenyang 110866) |
| |
| Abstract: | A pair of primers were designed according to the sequences of bovine viral diarrhea virus strain in public. Using these primers approximately 190 bp-long DNA products were amplified by RT-PCR from genetype I and genetype II products of bovine viral diarrhea virus,but not from other control samples. This method can detect as little as 0.10 ng bovine viral diarrhea virus RNA. |
| |
| Keywords: | Bovine viral diarrhea virus PCR diagnosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
