益气健脾中药修复腹泻仔猪小肠黏膜损伤的病理学观察

为了研究益气健脾中药对腹泻仔猪小肠黏膜组织形态及免疫细胞的影响,试验用抗生素及中药复方颗粒剂治疗腹泻仔猪,颈静脉放血处死后,腹腔解剖,无菌采集小肠黏膜,通过制作石蜡切片、HE染色观测小肠黏膜病理组织学变化;电镜包埋切片观察小肠黏膜细胞结构的变化。结果显示,中药复方颗粒剂治疗组小肠黏膜除有少量出血外,未发现明显病理变化。抗生素治疗组则小肠黏膜脱落,有广泛充血,出血;黏膜上皮细胞变性坏死;嗜酸性粒细胞浸润,有大量以淋巴细胞(LELs)、杯状细胞(GSs)为主的炎性细胞浸润;微绒毛排列紊乱,脱落;细胞受损严重,线粒体等细胞器结构异常。结果表明,抗生素治疗仔猪腹泻造成小肠黏膜显著的病理变化和功能障碍,而中药复方颗粒剂治疗有利于仔猪小肠黏膜结构的修复,为防治仔猪腹泻、提高仔猪肠道免疫功能提供理论依据。

Pathological Observation on the Repair of Small Intestinal Mucosa Injury in Diarrheal Piglets with Tonifying Qi-nourishing Spleen Herbs

To investigate the effect of Tonifying Qi-Nourishing Spleen herbs on Histomorphology and immune cells of small intestinal mucosa in diarrheal piglets,antibiotics and Chinese herbal medicine were adopted to heal diarrheal piglets.Piglets were sacrificed by bloodletting in veins,dissected peritoneal cavity,and gathered small intestinal mucosa abacterially.Then,the changes of histopathology and cellular structures in small intestinal mucosa were observed by using pathological and electron microscopic sections.The results showed that small intestinal mucosa had no obvious pathological changes except slight hemorrhage in Chinese herbal medicine group.Nevertheless,there were more pathological changes in antibiotics group.For instance,small intestinal mucosa had desquamated with extensive hyperemia and hemorrhage concomitantly;epithelial cells were degenerated and necrotic;eosinophilic granulocyte displayed cellular infiltration,which included inflammatory cell infiltrate principally as lymphocyte;microvillus had desquamated and arranged indiscriminately;mucous membrane cells were damaged seriously and mitochondria presented structural abnormalities.The study concluded that the therapy of using antibiotics in diarrheal piglets might cause pathological changes and functional impairment in small intestinal mucosa,and using Chinese herbal medicine might be beneficial for plerosis of small intestinal mucosa,which would provide the theoretical foundation to cure diarrhea and improve intestinal immune function in piglets.