| 拟南芥诱导型启动子rd29A的克隆及其功能鉴定 |
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| 引用本文： | 齐恩芳,贾小霞,马胜,文国宏,胡新元,龚成文,王一航,李建武.拟南芥诱导型启动子rd29A的克隆及其功能鉴定[J].干旱地区农业研究,2015,33(4):286-290. |
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| 作者姓名： | 齐恩芳 贾小霞 马胜 文国宏 胡新元 龚成文 王一航 李建武 |
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| 作者单位： | 甘肃省农业科学院马铃薯研究所，甘肃 兰州 730070; 甘肃省马铃薯种质资源创新工程实验室，甘肃 兰州 730070; 农业部西北旱作马铃薯科学观测实验站，甘肃 渭源 748201 |
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| 基金项目： | 甘肃省农业科学院中青年基金项目(2014GAAS20)；国家自然科学基金 |
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| 摘 要： | 以改善作物抗旱性为目的,采用PCR方法从拟南芥中克隆了诱导型启动子rd29A,序列分析发现克隆的rd29A启动子与已发表的rd29A启动子序列(D13044)的同源性为99.47%。利用DNA重组技术成功构建了rd29A启动子驱动GUS基因的植物表达载体p BI121-rd29-GUS,并通过农杆菌介导法转化烟草,转基因烟草叶片中GUS酶活性的组织化学检测结果表明,rd29A启动子能驱动目的基因的有效表达。因此,可以在后续的马铃薯抗旱转基因研究中直接应用。
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| 关 键 词： | rd29A启动子 抗逆性 GUS基因 |
Cloning and functional characterization of an inducible promoter rd29A from Arabidopsis thaliana |
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| QI En-fang,JIA Xiao-xi,MA Sheng,WEN Guo-hong,HU Xin-yuan,GONG Cheng-wen,WANG Yi-hang,LI Jian-wu.Cloning and functional characterization of an inducible promoter rd29A from Arabidopsis thaliana[J].Agricultural Research in the Arid Areas,2015,33(4):286-290. |
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| Authors: | QI En-fang JIA Xiao-xi MA Sheng WEN Guo-hong HU Xin-yuan GONG Cheng-wen WANG Yi-hang LI Jian-wu |
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| Abstract: | To improve drought resistance of crops, inducible promoter rd29A was cloned from Arabidopsis thaliana by PCR method. Sequencing analysis indicated that the cloned fragment showed 99.47% identity to the published sequence (D13044). An rd29a driving GUS expression vector pBI121-rd29-GUS was constructed by DNA recombinant technology. By Agrobacterium-mediated transformation, pBI121-rd29-GUS was transformed into tobacco. The function of rd29A promoter was identified through the expression of GUS protein in transgenic tobacco. GUS activity in transgenic tobacco leaf showed that the rd29A promoter could drive efficient expression of the target gene. rd29A promoter could be used in subsequent transgenic study of drought resistance in potato. |
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| Keywords: | rd29A promoter stress resistance GUS gene |
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