| 簇毛麦端体6VS的抗病同源序列的克隆及分析 |
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| 引用本文： | 孔凡晶,马有志,陈孝,辛志勇. 簇毛麦端体6VS的抗病同源序列的克隆及分析[J]. 中国农业科学, 2003, 36(10): 1223-1227 |
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| 作者姓名： | 孔凡晶 马有志 陈孝 辛志勇 |
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| 作者单位： | 1. 中国农业科学院作物育种栽培研究所,北京,100081;国土资源部盐湖资源与环境开放实验室,北京,100037
2. 中国农业科学院作物育种栽培研究所,北京,100081 |
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| 基金项目： | 国家“863”资助项目(Z-17-04-01)，植物转基因专项及产业化资助项目(J00-A-002) |
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| 摘 要： | 本研究采用玻璃针切割法对小麦遗传背景下的簇毛麦端体6VS进行了微切割,根据已克隆抗病基因的保守域设计引物,从6VS端体DNA中扩增出10类抗病基因同源片段Hvrgak1-Hvrgak10(GenBank登录号为AF387113-AF387120,AY040671,AY040672)。并利用抗病同源序列作探针,进行与小麦抗白粉病基因的相关分析,结果表明,Hvrgak2、Hvrgak4和Hvrgak5可能与抗白粉病基因相关。这些抗病基因同源片段可以为抗病基因克隆提供切入点或是直接的探针。
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| 关 键 词： | 簇毛麦端体6VS 微切割 抗病基因同源片段 克隆 |
| 修稿时间： | 2002-11-19 |
Cloning and Characterization of a Family of Disease Resistance Gene Analogs from 6VS of Haynaldia villosa |
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| KONG Fan-jing,MA You-zhi,CHEN Xiao,XIN Zhi-yong. Cloning and Characterization of a Family of Disease Resistance Gene Analogs from 6VS of Haynaldia villosa[J]. Scientia Agricultura Sinica, 2003, 36(10): 1223-1227 |
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| Authors: | KONG Fan-jing MA You-zhi CHEN Xiao XIN Zhi-yong |
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| Abstract: | Microdissection of 6VS and the cloning of the resistance gene analogs (RGA) from 6VS of H. villosa were studied. The 6VS were microdissected with needle and 10 types of resistance gene analogs were obtained by PCR with degenerate oligonucleotide primer designed according to resistance genes. They were designated as Hvrgakl-Hvr-gak10, and the GenBank accession numbers are AF387113-AF387121, AY040671-AY040672. Identity among RGAs was about 10% - 50% , and identity with cloned R gene from plants was 5% - 20% . Southern hybridization analysis results showed 3 RGAs, Hvrgak2, Hvrgak4, and Hvrgak5 were linked with wheat powdery mildew resistance. These RGAs may be used as direct entrance or probes for cloning the disease resistance genes. |
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| Keywords: | 6VS of Haynaldia villosa Microdissection Resistance gene analogs (RGA) Cloning |
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