| 小麦矮秆种质系山农495矮秆基因的分子标记定位 |
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| 引用本文： | 宗浩,崔法,鲍印广,赵春华,王玉海,杜斌,王庆专,王洪刚.小麦矮秆种质系山农495矮秆基因的分子标记定位[J].麦类作物学报,2009,29(3). |
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| 作者姓名： | 宗浩 崔法 鲍印广 赵春华 王玉海 杜斌 王庆专 王洪刚 |
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| 作者单位： | 山东农业大学作物生物学国家重点实验室/国家小麦改良中心泰安分中心/山东农业大学农学院,山东泰安,271018 |
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| 基金项目： | 山东省农业良种工程重大课题 |
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| 摘 要： | 为给新矮秆种质系山农495的利用提供依据,利用田间观察和室内赤霉酸鉴定与分子标记分析相结合的方法,进行矮秆性状的遗传分析和矮秆基因分子标记定位.结果表明,山农495的矮秆性状受位于4BS染色体上的1对隐性主效基因控制,且为赤霉酸不敏感型.在供试的1 589个SSR引物中,有2个基因组SSR引物(Gwm 113196、Wmc 511191)和1个EST-SSR引物(Dupw 23210)在优选小群体中能扩增出多态性谱带.利用BC1回交群体和F2群体进行连锁标记分析,Gwm 113196、Wmc 511191和Dupw 23210与山农495矮秆基因的连锁距离分别为3.9、5.2、21.6 cM和4.1、5.0、19.8 cM.
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| 关 键 词： | 小麦 山农495 矮秆基因 分子标记定位 |
Developing Molecular Markers for the Rht Gene in Dwarfing Germplasm Line Shannong 495 |
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| ZONG Hao,CUI F,BAO Yin-guang,ZHAO Chun-hu,WANG Yu-hai,DU Bin,WANG Qing-zhuan,WANG Hong-gang.Developing Molecular Markers for the Rht Gene in Dwarfing Germplasm Line Shannong 495[J].Journal of Triticeae Crops,2009,29(3). |
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| Authors: | ZONG Hao CUI F BAO Yin-guang ZHAO Chun-hu WANG Yu-hai DU Bin WANG Qing-zhuan WANG Hong-gang |
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| Abstract: | In this study,the genetic characteristic of Rht gene in Shannong 495 was studied by field investigation,GA reaction identification and molecular marker.The results showed that the dwarfing gene in Shannong 495 may be a recessive gene on chromosome 4BS;this dwarfing gene was insensitive to GA;1 589 genomic SSR and EST-SSR markers were screened for polymorphism in the Preferred Small Group(PSG) and two genomic SSR markers(Gwm 113196 and Wmc 511191) and one EST-SSR marker(Dupw 23210) were identified polymorphi... |
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| Keywords: | Wheat Shannong 495 Dwarf gene SSR EST-SSR PSG |
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