14-3-3σ negatively regulates Akt and inhibits Rat1-Akt cell xenografts |
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Authors: | XIA Hong-ping YANG Hui-ling XIA Yun-fei HUANG Wen-lin LEE Mong-hong SU Yong |
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Institution: | 1.Department of Pathophysiology, Sun Yat-sen University, Guangzhou 510080, China. E-mail:yanghl@mail.sysu.edu.cn;2.Cancer Center of Sun Yat-sen University, Guangzhou 510060, China;3.The University of Texas M.D. Anderson Cancer Center, TX 77030, USA |
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Abstract: | AIM:The present study was designed to investigate the effect of adenovirus-mediated 14-3-3σ on Rat1-Akt cell xenografts and to explore whether the effect was mediated through negative regulation of Akt. METHODS:The effect of Ad-14-3-3σ on Rat1-Akt cells xenografts was observed in nude mice ex vivo and in vivo. Western blotting was used to detect 14-3-3σ protein,phospho-Akt (Thr 308), phospho-Akt (Ser 473), and phospho-(Ser/Thr) Akt substrate in tumor tissue after transfection of 14-3-3σ gene. RESULTS:The tumor volume was dramatically decreased and its emergence was delayed, regardless of using Ad-14-3-3σ-treated Rat1-Akt cells (ex vivo) or injected Ad-14-3-3σ in vivo, of which the effect of the continuously injected group was the best. Levels of Akt protein, phosphorylated Akt and phosphorylated Akt substrates in tumors obtained from Ad-14-3-3σ-treated mice were markedly less than those in PBS or Ad-β-gal-treated mice. CONCLUSION:14-3-3σ suppressed Akt overexpession in cells of Rat1-Akt xenografts by negatively regulating Akt. |
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