Effects of heat shock protein 70 on inflammation after hepatic local ischemia/reperfusion in rats

AIM:To study the effects of heat shock protein 70 (HSP70) induced by the heat stress pretreatment on inflammation after hepatic ischemia/resperfusion.METHODS:With the hepatic local ischemia/reperfusion model (IR group),heat stress pretreatment (H+IR group) and injecting quercetin before heat stress pretreatment (Q+H+IR group) were performed.The HSP70,intercellular adhesion molecule-1 (ICAM-1) and the myeloperoxidase (MPO) activity were detected.The levels of serum ALT and AST and histological changes of the hepatocytes were also observed.RESULTS:In H+IR group,the HSP70 expression was higher than that in other groups at each time point,after performing ischemia-perfusion,hepatic injury was slighter.The levels of serum ALT and AST were increased slightly (P<0.01).The expression of ICAM-1 and the changes of MPO activity increased and peaked respectively at 6 h and 12 h after reperfusion.However,they were lower in H+IR group than those in IR group and Q+H+IR group.CONCLUSION:The HSP70 induced by heat stress pretreament reduces the expression of ICAM-1 and the changes of MPO activity during hepatic ischemia-reperfusion,then hepatic injury is depressed from the inflammation.