Effect of TLR4 activation on endothelium adhesion function and atorvastatin intervention |
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Authors: | WANG Hong-yan QU Peng YU Zhi-hong JIANG Hua ZHOU Xiao-yu |
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Institution: | 1.Department of Cardiology,2 Center Laboratory,The Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China;3.The Center Laboratory,Zhongxin Hospital of Dalian,Dalian 116000,China |
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Abstract: | AIM:To investigate the modulation of LOX-1 and monocyte-endothelium adhesion by TLR4 activation in human umbilical vein endothelial cells (HUVECs),and explore LOX-1’s role in mediating monocyte-endothelium adhesion,and the effect of atorvastatin.METHODS:TLR4 and LOX-1 mRNA were measured by RT-PCR.The expression percentage of TLR4 and LOX-1 positive cells were detected by flow cytometry.The adhesive percentage between monocytes and HUVECs were determined by counting.RESULTS:Incubation by LPS (1 mg/L) for 24 hours upregulated TLR4,LOX-1 mRNA and protein expression in HUVECs,and increased the percentage of monocyte adhesion to endothelium.Pretreatment of cell with anti-LOX-1 partly abolished the increase of monocyte adhesion to endothelium.Atorvastatin (10 μmol/L) inhibited LPS-mediated effects above.CONCLUSION:TLR4 activation upregulates LOX-1 expression and increases monocyte-endothelium adhesion.LOX-1 partly involves in LPS-induced monocyte-endothelium adhesion,atorvastatin may have protective effects on endothelium by inhibiting TLR4 and TLR4-induced LOX-1 expression. |
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