Inter and intra-species Inter Simple Sequence Repeat (ISSR) variations in the genus Cicer |
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Authors: | Mehmet Ali Sudupak |
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Institution: | (1) Department of Biology, Faculty of Arts and Sciences, Middle East Technical University, Ankara, Turkey |
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Abstract: | Intra and inter-species ISSR variation and use of ISSR markers in determination of genetic relationship were investigated
in an accession collection representing twoperennial and six annual Cicerspecies. Screening of Ciceraccessions with SSR primers revealed highly reproducible amplicon profiles with relatively high multiplex ratios. Many of
the primers generated amplicon profiles with which not only the differences among species can readily be identified, but also
polymorphisms within species could be detected more efficiently. PCR products at 150 gel positions detected using six SSR
primers in Cicer accessions were treated as dominant DNA markers and utilized to compute the distances among accessions and species. Cluster
analysis of accessions and species revealed groupings that corroborate our previous studies of relationships based on allozyme
and AFLP analysis. Consistent with the AFLP analysis carried out in the same accession collection, ISSR-based groupings indicated
that perennial C. incisumis genetically close to the annuals of the second crossability group (C. pinnatifidum,C. bijugum, C. judaicum) while C. reticulatum is the closest wild species to the cultivated chickpea. ISSR-based variation estimates were relatively higher when compared
to previous estimates computed from RAPD and AFLP data. Technically, ISSR analysis combines the PCR-based targeting of microsatellite-associated
polymorphisms with no prior sequence requirement and stringent PCR conditions. Similarly, when compared to AFLP analysis,
it is less technically demanding allowing to survey polymorphic loci in the genome. Thus, ISSR-PCR technology is a reliable,
fast, and cost-effective marker system that can be used to study genetic variation and genetic relationships in the genusCicer.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Cicer chickpea DNA variation microsatellite species relationship |
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