| 磁性纳米颗粒负载质粒DNA的研究 |
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| 引用本文： | 彭子艾,李丹丹,夏澳运,王加峰,黄翠红,王慧,郭涛,陈志强. 磁性纳米颗粒负载质粒DNA的研究[J]. 华南农业大学学报, 2020, 41(1): 78-82. DOI: 10.7671/j.issn.1001-411X.20190518 |
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| 作者姓名： | 彭子艾 李丹丹 夏澳运 王加峰 黄翠红 王慧 郭涛 陈志强 |
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| 作者单位： | 华南农业大学农学院/国家植物航天育种工程技术研究中心,广东广州510642;华南农业大学农学院/国家植物航天育种工程技术研究中心,广东广州510642;华南农业大学农学院/国家植物航天育种工程技术研究中心,广东广州510642;华南农业大学农学院/国家植物航天育种工程技术研究中心,广东广州510642;华南农业大学农学院/国家植物航天育种工程技术研究中心,广东广州510642;华南农业大学农学院/国家植物航天育种工程技术研究中心,广东广州510642;华南农业大学农学院/国家植物航天育种工程技术研究中心,广东广州510642;华南农业大学农学院/国家植物航天育种工程技术研究中心,广东广州510642 |
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| 基金项目： | 广东省重点领域研发计划（2018020206002）；国家重点研发计划（2016YFD0102102）；国家水稻产业技术体系建设专项（CARS-01-17） |
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| 摘 要： | 【目的】研究磁性纳米颗粒(MNP)负载质粒DNA形成的纳米载体-基因复合物的生物物理学特性、负载形态与负载机理,为基于磁性纳米载体定向编辑技术的应用奠定基础。【方法】以MNP作为基因载体,与质粒DNA pRGEB32制备纳米载体-基因复合物MNP-pRGEB32,分析MNP负载、保护pRGEB32的能力,并对其粒度分布、Zeta电位、结合形态进行研究。【结果】MNP通过静电作用压缩、吸附、聚集pRGEB32,形成纳米载体-基因复合物。MNP能有效负载并保护pRGEB32。【结论】MNP可作为一种理想的基因转移载体。
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| 关 键 词： | 磁性纳米颗粒 纳米载体-基因复合物 负载形态 负载机理 |
| 收稿时间： | 2019-05-10 |
Study on magnetic nanoparticle loading plasmid DNA |
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| PENG Ziai,LI Dandan,XIA Aoyun,WANG Jiafeng,HUANG Cuihong,WANG Hui,GUO Tao and CHEN Zhiqiang. Study on magnetic nanoparticle loading plasmid DNA[J]. JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY, 2020, 41(1): 78-82. DOI: 10.7671/j.issn.1001-411X.20190518 |
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| Authors: | PENG Ziai LI Dandan XIA Aoyun WANG Jiafeng HUANG Cuihong WANG Hui GUO Tao CHEN Zhiqiang |
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| Affiliation: | College of Agriculture, South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China,College of Agriculture, South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China,College of Agriculture, South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China,College of Agriculture, South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China,College of Agriculture, South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China,College of Agriculture, South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China,College of Agriculture, South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China and College of Agriculture, South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China |
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| Abstract: | Objective To study biophysical property, loading morphology and loading mechanism of nanocarrier-gene complex prepared by magnetic nanoparticle (MNP) loading plasmind DNA, and lay a foundation for application of oriented editing technology based on magnetic nano carrier.Method MNP was used as gene carrier to prepare nanocarrier-gene complex MNP-pRGEB32 with plasmid DNA pRGEB32. The abilities of MNP loading and protecting pRGEB32 were analyzed. The particle size distribution, Zeta potential and loading morphology of MNP-pRGEB32 were investigated.Result MNP compressed, adsorbed and aggregated pRGEB32 through electrostatic interaction to form nanocarrier-gene complex. MNP could effectively load and protect pRGEB32.Conclusion MNP can be used as an ideal gene transfer carrier. |
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| Keywords: | magnetic nanoparticle nanocarrier-gene complex loading morphology loading mechanism |
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