猪伪狂犬病毒H株gE基因的克隆及序列分析

参照已发表的扩增伪狂犬病毒gI和gE部分基因的PCR方法,合成了一对引物,以PRV H株细胞毒为模板,扩增出一条约850 bp的片段,并进行克隆和测序。然后与GenBank上国内外其它毒株gE基因进行核苷酸和推导的氨基酸序列的同源性分析,构建了遗传进化关系图。结果表明,PRV H株与其他15株PRV核苷酸和氨基酸的同源性分别为97.0%~99.2%和93.2%~98.5%,其中与SH株最近,分别为99.2%和98.5%;进化树分析表明,该毒株与目前国内流行的毒株在同一进化分支内,与国外毒株分属不同分支,说明分离的H毒株与目前国内流行的毒株一致。

Cloning and Sequence Analysis of gE Gene Fragment of Pseudorabies Virus H Strain

According to the gene gI and gE clone method,the PCR primer was synthesized,a fragment of 850 bp was carried out,and then PCR product was cloned and sequenced.Then compared others PRV strains,the nucleotide sequence,deduced amino acid sequence homology analysis and the cladogram were made.The result indicated that the nucleotides and amino acids identity of PRV gE gene was 97.0% to 99.3% and 93.2% to 98.5% comparing with other 15 PRV isolates published in GenBank,and with 99.2% nucleotides identity and 98.5...