8种柑橘类植物对柑橘碎叶病毒分子组成的影响

探究不同柑橘类型对柑橘碎叶病毒(Citrus tatter leaf virus,CTLV)变异的影响,为研究寄主-CTLV的互作关系提供基础。将CTLV分离株HH和XLB分别嫁接接种于8种柑橘类植物,对CTLV分离株和获得的亚分离株样品进行CP基因的限制性长度多态性(RFLP)和单链构象多态性(SSCP)分析,以及序列比较。结果表明HH和XLB与其各自在不同柑橘植株上的亚分离株的CP/Hinf I RFLP酶切图谱无明显差异;混合侵染的CTLV分离株HH和其亚分离株之间的SSCP谱型存在差异,株系构成单一的CTLV分离株XLB与其亚分离株间的SSCP谱型差异不明显。通过序列分析进一步证实CTLV在不同寄主上其CP基因有几个核苷酸位点发生了变异。CTLV可能受寄主植物的影响发生了变异。

Influence of eight citrus cultivars on the composition of Citrus tatter leaf virus

Citrus tatter leaf disease caused by Citrus tatter leaf virus(CTLV) is an economically important systemic disease of citrus in the world.It is known that CTLV is widespread in China,and it has caused tremendous economic loss in Zhejiang,Hunan,Fujian and Guangxi provinces.So it is important to understand the influence of citrus cultivars on the composition of CTLV.48 sub-isolates of CTLV isolates HH and XLB were obtained by inoculating HH and XLB to Jincheng,Lemon,Ponkan,Tangerine,Daidai,None,Fragrant orange and Rusk citrange.Then restriction fragment length polymorphism(RFLP),single-strand conformation polymorphism(SSCP) and sequence analysis were applied to analyze the variation of HH,XLB and their sub-isolates.The results showed that the RFLP patterns of coat protein gene(CP) digested by restriction enzyme Hinf I of CTLV isolate HH,XLB and their sub-isolates were similar.Based on SSCP analysis,HH mixed with several strains changed its polymorphism in some hosts.However,XLB with single strain did not change its polymorphism in different citrus hosts.In sequence analysis,it was found that the nucleotide identity in the CP gene of isolate HH and its sub-isolates was 99.3% to 100%,and that of isolate XLB and its sub-isolates was 96.5% to 99.8%.The results indicated that the composition of CTLV isolates might be changed in different citrus hosts.