鸡血藤总黄酮对大肠杆菌败血症的治疗作用

为研究鸡血藤总黄酮对小鼠大肠杆菌败血症的治疗作用,本研究采用腹腔注射大肠杆菌菌液的方法建立小鼠大肠杆菌败血症模型,1 h后灌胃给予不同剂量鸡血藤总黄酮,12 h后观察小鼠临床表现并进行评分,然后剖杀小鼠,比较肝脏、胸腺和脾脏的外观、脏器指数和肝脏病理切片结果,检测各项血液常规指标,分析血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)及乳酸脱氢酶(LDH)水平,测定血清中白细胞介素1β(IL-1β)、IL-2、IL-6、IL-10及肿瘤坏死因子α(TNF-α)等炎性细胞因子的水平。结果发现,腹腔注射大肠杆菌后引起小鼠被毛粗乱、精神沉郁、眼睛红肿、眼部分泌物增加且呼吸急促等症状,部分严重的小鼠出现抽搐、休克现象,肝脏、胸腺、脾脏出现萎缩、变小的现象,且脏器指数下降。肝脏病理切片可观察到肝索紊乱、肝窦增大、细胞边缘不清晰、部分区域炎性细胞浸润。血液中白细胞(WBC)、淋巴细胞(LYM)、血红蛋白(HGB)、红细胞(RBC)和血小板(PLT)等指标水平发生显著改变(P<0.05),且血清中ALT、AST、LDH及炎性细胞因子等水平显著升高(P<0.05)。而鸡血藤总黄酮和阿米卡星处理可改善上述临床表现,抑制相关指标的改变,使其趋于与对照组相似水平。以上结果表明,鸡血藤总黄酮可通过提高机体免疫水平及抑制炎性反应减轻大肠杆菌败血症引起的机体损伤。

Therapeutic Effect of Total Flavonoid of Spatholobus suberectus Dunn on Escherichia coli Sepsis

In order to evaluate the therapeutic effect of total flavonoids from Spatholobus suberectus Dunn (TFSD) on Escherichia coli (E.coli) sepsis,sepsis animal models were established in mice by intraperitoneally injected with E.coli,mice were administrated with various dose of TFSD after 1 h,the clinical manifestations of mice were observed and scored after 12 h.Then the mice were dissected,the liver,thymus and spleen were observed and weighted for the calculation of their organ indexes,pathological sections of livers were prepared for detail analysis of pathological change.Blood routine indicators were measured to analyze the levels of aspartate aminotransferase (AST),alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) in serum.The levels of inflammatory cytokines such as interleukin-1β (IL-1β),IL-2,IL-6,IL-10 and tumor necrosis factor α (TNF-α) in serum were determined.The results showed that the injection of E.coli resulted in irregular hair coat,poor spirit,inflamed eyes,increase of secretion from eyes,tachypnea,even convulsion or shock.Atrophy was observed from liver,thymus and spleen tissues,corresponding with reduced organ index.Pathological sections indicated disorder of hepatic cord,swell of hepatic sinus,fuzziness of cell edges,and infiltration of inflammatory cells.The concentrations of leukocytes (WBC),lymphocyte (LYM),hemoglobin (HGB),red blood cell (RBC) and platelet (PLT) were significantly altered by the injection of E.coli (P<0.05).The levels of ALT,AST,LDH and inflammatory cytokines were significantly increased (P<0.05).However,the treatment of TFSD and amikacin was able to alleviate the clinical manifestation and inhibit the change of organ indexes,which tended to be similar to the control group.The results indicated that TFSD was able to alleviate E.coli induced injury by improving the immune response and inhibit inflammatory reaction in mice.