| 天津地区猪链球菌3型菌株的致病性及耐药特性研究 |
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| 引用本文： | 池晶晶,董志民,田向学,谭世旭,李秀丽,杨春蕾,任卫科,鄢明华.天津地区猪链球菌3型菌株的致病性及耐药特性研究[J].中国畜牧兽医,2019,46(12):3740-3748. |
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| 作者姓名： | 池晶晶 董志民 田向学 谭世旭 李秀丽 杨春蕾 任卫科 鄢明华 |
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| 作者单位： | 1. 天津市畜牧兽医研究所, 天津 300381;2. 农业农村部兽用药物与诊断技术天津科学观测实验站, 天津 300381;3. 天津市畜禽健康养殖技术工程中心, 天津 300381 |
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| 基金项目： | 天津市生猪产业技术体系猪病防控岗位项目（ITTPRS2017003）；天津市科技计划项目（18ZXBFNC00270） |
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| 摘 要： | 试验旨在对分离自天津地区发病猪场的7株猪链球菌3型菌株（Streptococcus suis type 3，SS 3）进行致病性和耐药特性研究。应用剂量为1.0×107、1.0×108和1.0×109 CFU/只的细菌对小鼠进行致病力研究，用PCR方法检测7株SS 3型菌株的毒力基因mrp、ef、sly、gdh、gapdh、fbps和orf2，并对这7株SS 3型菌株进行药物敏感试验。致病力试验结果显示，接种剂量为1.0×109和1.0×108 CFU/只时分别有6和3株SS 3型菌株可使小鼠100.0%（5/5）发病死亡，接种剂量1.0×107 CFU时仍有1株SS 3型菌株可使80.0%（4/5）小鼠发病死亡，这7株SS 3型菌株对小鼠的致病力依次为R15056 > R15042=S15030 > Y12024=Y09011 > Y13125 > Y13164。毒力基因检测结果表明，共有3种毒力基因型，R12024、Y13164、R15042和S15030株为gdh、gapdh、fbps和orf2毒力基因阳性，Y13125和R15056株为sly、gdh、gapdh、fbps和orf2毒力基因阳性，Y09011株为gdh、fbps和orf2毒力基因阳性。药物敏感性试验结果显示，7株SS 3型对头孢喹肟相对最敏感，其次为阿莫西林、环丙沙星和磺胺间甲氧嘧啶钠，但对强力霉素100.0%耐药，且所有菌株均呈现3重以上的耐药性。本研究为进一步开展天津地区SS 3型流行特点及致病机理研究奠定了基础，可为天津地区SS 3型菌株的综合防控提供理论指导。
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| 关 键 词： | 猪链球菌3型 鉴定 致病力 毒力基因 药物敏感试验 |
| 收稿时间： | 2019-07-12 |
Study on Pathogenicity and Drug Resistance of Streptococcus suis Type 3 Strain in Tianjin |
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| CHI Jingjing,DONG Zhimin,TIAN Xiangxue,TAN Shixu,LI Xiuli,YANG Chunlei,REN Weike,YAN Minghua.Study on Pathogenicity and Drug Resistance of Streptococcus suis Type 3 Strain in Tianjin[J].China Animal Husbandry & Veterinary Medicine,2019,46(12):3740-3748. |
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| Authors: | CHI Jingjing DONG Zhimin TIAN Xiangxue TAN Shixu LI Xiuli YANG Chunlei REN Weike YAN Minghua |
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| Institution: | 1. Tianjin Animal Science and Veterinary Research Institute, Tianjin 300381, China;2. Tianjin Scientific Observation Experiment Station of Veterinary Medicine and Diagnosis Technology, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Tianjin 300381, China;3. Tianjin Engineering Research Center for Livestock and Poultry Health Breeding, Tianjin 300381, China |
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| Abstract: | The aim of this study was to investigate the pathogenicity and drug resistance of 7 Streptococcus suis type 3 (SS 3) strains isolated from piggery in Tianjin.The pathogenicity of bacteria with doses of 1.0×107,1.0×108 and 1.0×109 CFU/mice was studied.The virulence genes mrp,ef,sly,gdh,gapdh,fbps and orf2 of 7 strains of SS 3 strains were detected by PCR.Drug susceptibility tests of the 7 strains of SS 3 were also carried out.The results of pathogenicity test showed that 6 and 3 strains of SS 3 could cause 100.0% (5/5) morbidity and death in mice at inoculation dose of 1.0×109 and 1.0×108 CFU/mice,respectively.One strain of SS 3 could still cause 80.0% (4/5) morbidity and death in mice at inoculation dose of 1.0×107 CFU/mice.The order of pathogenicity of the 7 strains of SS3 strains in mice was R15056 > R15042=S15030 > Y12024=Y09011 > Y13125 > Y13164.The results of virulence gene test showed that there were three virulence genotypes:R12024,Y13164,R15042 and S15030 were positive for gdh,gapdh,fbps and orf2 virulence genes;Y13125 and R15056 were positive for sly,gdh,gapdh,fbps and orf2 virulence genes,and Y09011 was positive for gdh,fbps and orf2 virulence genes.Drug susceptibility test showed that 7 SS 3 strains were relatively sensitive to cefquinoxime,followed by amoxicillin,ciprofloxacin and sulfamethoxacil sodium.However,they showed 100.0% resistance to doxycycline,and all strains showed more than 3-fold resistance.This study laid a foundation for the further development of epidemiological characteristics and pathogenic mechanism of SS 3 strains in Tianjin,and could provide theoretical guidance for the comprehensive prevention and control of SS 3 strains in Tianjin. |
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| Keywords: | Streptococcus suis type 3 identification pathogenicity virulence gene drug sensitivity test |
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