| RT-PCR克隆广谱抗病基因NPR1及其蛋白表达载体构建 |
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| 引用本文： | 刘永光,刘克锋,孙向阳.RT-PCR克隆广谱抗病基因NPR1及其蛋白表达载体构建[J].农业科学与技术,2011(6):852-854,930. |
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| 作者姓名： | 刘永光 刘克锋 孙向阳 |
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| 作者单位： | 北京林业大学水保学院;北京农学院城乡发展学院 |
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| 基金项目： | Supported by project of Beijing Municipal Education Commission(KM200910020014);Project of Sand Control Department,Beijing Municipal Landscape Greening Bureau(2008)~~ |
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| 摘 要： | 目的]克隆植物广谱抗病基因NPR1并构建其蛋白表达载体。方法]提取拟南芥总RNA,设计相关引物,采用反转录PCR方法克隆NPR1基因;利用酶切连接方法,将该基因正向导入蛋白表达载体。结果]经过相关检验,将NPR1正向插入pMXB10载体中,得到了pMXB10-NPR1蛋白表达载体。结论]成功构建了包含NPR1的蛋白表达载体。
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| 关 键 词： | NPR1 广谱抗病 载体构建 |
Cloning of Broad-spectrum Anti-disease NPR1 Gene with RT-PCR and Construction of Its Protein Expression Vector |
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| LIU Yong-guang,LIU Ke-feng,SUN Xiang-yang.Cloning of Broad-spectrum Anti-disease NPR1 Gene with RT-PCR and Construction of Its Protein Expression Vector[J].Agricultural Science ＆ Technology,2011(6):852-854,930. |
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| Authors: | LIU Yong-guang LIU Ke-feng SUN Xiang-yang |
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| Institution: | 1.School of Soil and Water Conservation,Beijing Forestry University,Beijing 100083;2.College of Urban and Rural Development,Beijing University of Agriculture,Beijing 102206 |
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| Abstract: | |
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| Keywords: | Nonexpressor of pathogenesis-related genes 1(NPR1) Broad-spectrum anti-disease Construction of vectors |
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