| 山羊红细胞膜表面CD58的制备与鉴定 |
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| 引用本文： | 靳亚平,王建辰.山羊红细胞膜表面CD58的制备与鉴定[J].动物医学进展,2000,21(2):54-56. |
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| 作者姓名： | 靳亚平 王建辰 |
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| 作者单位： | 西北农林科技大学动物科学与动物医学学院,陕西杨陵 |
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| 基金项目： | 国家自然科学基金资助项目! (39770 5 45 ) |
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| 摘 要： | 应用胰蛋白酶消化、三氯乙酸沉淀、透析等方法，提取山羊ＲＢＣ同ＣＤ５８。玫瑰花环抑制试验表明，８．０ｇ／Ｌ提取物对猪淋巴细胞的Ｅｔ花环抑制率达到 ６８．６％，且该活性可被ＣＤ２封闭；淋巴细胞转化试验证明，提取物能刺激活化猪ＰＢＭＣ，与绵羊ＲＢＣ源ＣＤ５８的作用间无显著差异（Ｐ〉０．０５），经ＰＢＬ吸收后，其活性显著降低（Ｐ〈０．０１）。
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| 关 键 词： | 山羊 PBMC活化 CD58 提取 鉴定 Et花环抑制率 |
The Preparation and identification of CD58 from Goat RBC |
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| JIN Ya ping,WANG Jian chen WANG Ai hua.The Preparation and identification of CD58 from Goat RBC[J].Progress In Veterinary Medicine,2000,21(2):54-56. |
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| Authors: | JIN Ya ping WANG Jian chen WANG Ai hua |
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| Abstract: | The CD58 of goat RBC was separated with method of trypsin digestion ,deposition with chloroactic acid and dilysis.The Et rosseting inhibition test indicated that the Et rosseting inhibition ration in 8.0 g of the preparation to pig PBMC reached 68.6%,this activation was able to be blocked out by CD2 ,the lymphocyte transform test proved that the preparation was able to induce the pig PBMC to proliferation ,and there is no significant difference with the SRBC derived CD58 (P>0.05) ,the activation reduced significantly after absorbed with PBL( P<0.01). |
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| Keywords: | goat CD58 identification |
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