| 啤酒发酵过程中野生酵母污染检验新方法研究 |
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| 引用本文： | 何培彦,吴雪昌. 啤酒发酵过程中野生酵母污染检验新方法研究[J]. 农机化研究, 2007, 0(3): 95-97 |
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| 作者姓名： | 何培彦 吴雪昌 |
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| 作者单位： | 浙江大学,生命科学学院,杭州,310058 |
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| 摘 要： | 提取培养酵母TY-1和野生酵母W1,W2的基因组DNA,进行Delta-PCR,可以分别得到稳定而独特的DNA指纹图谱.把TY-1,W1和W2按比例混合,提取混合菌基因组DNA,进行Delta-PCR.结果表明:混合菌的Delta-PCR指纹图谱是纯菌图谱的叠加.通过对凝胶电泳图谱中特征带的分析,可以看出:即使混合菌体系中仅含有0.05%的野生酵母也能被Delta-PCR技术检出.
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| 关 键 词： | 食品工业 啤酒发酵 试验 Delta-PCR 野生酵母 啤酒发酵过程 野生酵母 污染检验 新方法研究 Process Contamination Inspection 体系 分析 特征 凝胶电泳图谱 叠加 结果 混合菌 比例混合 指纹图谱 稳定 基因组 培养酵母 提取 |
| 文章编号： | 1003-188X（2007）03-0095-03 |
| 收稿时间： | 2006-05-30 |
| 修稿时间： | 2006-05-30 |
New Method for Inspection of Contamination Yeasts in the Process of Brewage |
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| HE Pei-yan,WU Xue-chang. New Method for Inspection of Contamination Yeasts in the Process of Brewage[J]. Journal of Agricultural Mechanization Research, 2007, 0(3): 95-97 |
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| Authors: | HE Pei-yan WU Xue-chang |
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| Affiliation: | College of Life Science, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | Genomic DNA specific primers of delta of the three yeast strains including TY-1, W1 and W2 were amplified by PCR with sequence. Each strain showed the stable and unique DNA fingerprint when PCR products were analyzed in gel electrophoresis. TY-1, W1 and W2 were mixed proportionately, and examined by Delta-PCR. The result showed that the DNA fingerprints of the mixture were the superposition if each pure yeast strains. Analysis of the main bands showed that contamination yeast can be examined by Delta-PCR even if the mixed yeast system contained 0.05% of contamination yeast. |
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| Keywords: | food industry brewage yeast experiment Delta-PCR contamination yeast |
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