Activity and degradation of streptomycin and cycloheximide in soil |
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Authors: | L Badalucco F Pomaré S Grego L Landi P Nannipieri |
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Institution: | (1) Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S. Camillo De Lellis, I-01100 Viterbo, Italy;(2) Dipartimento di Scienza del Suolo e Nutrizione della Pianta, P. le delle Cascine 15, I-50144 Firenze, Italy |
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Abstract: | Streptomycin and cycloheximide were added (3 and 2 mg g-1 dry soil, respectively) single and in combination to a forest soil to follow their possible degradation and their effects on soil mineralization-immobilization processes. After 0, 1, 2, 4, 7, and 10 days of incubation at 25°C and 60% water-holding capacity, measurements were taken of microbial biomass C and N, the evolution of CO2, exchangeable NH
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, 0.5M K2SO4-extractable organic C, and total N in both unfumigated and CHCl3-fumigated soil. The results indicated that during the first 2 days of incubation, soil microorganisms were killed by the antibiotics and/or by CHCl3 and used subsequently as a substrate by the survivors. Thereafter, surviving microorganisms probably also started to use biocidal molecules as an energy and nutrient source. The ratios of biomass C to biomass N and of CO2 evolved to net NH
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produced indicated that both biocides had non-target effects for most of the incubation. Thus, streptomycin and cycloheximide are not suitable in determining the relative contribution from fungi and bacteria to mineralization-immobilization processes in soils. |
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Keywords: | Streptomycin Cycloheximide Selective inhibition Mineralization-immobilization Non-target effects Biocide degradation |
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