云南杂交玉米SSR标记核心引物的筛选及多样性分析

为降低合成引物成本,加快杂交玉米品种鉴定进程,筛选适用于云南玉米杂交种SSR标记鉴定的核心引物。本研究用玉米SSR标记鉴定规程中推荐的40对SSR引物对12个杂交玉米品种DNA进行扩增,筛选出多态性高、扩增效果好、稳定性好的20对引物,推荐其作为云南玉米杂交种特异性鉴定的核心引物。并用筛选的核心引物对云南220个杂交玉米材料进行多样性分析及评价。筛选的20对SSR引物在12个杂交玉米品种中共检测到216个等位变异,平均每对引物检测到10.80个。20对SSR核心引物在220个杂交玉米材料中共检测到236个等位变异,平均每对引物检测到11.80个;平均有效等位变异为3.158 8;平均Shannon-Weaver多样性信息指数为3.028 2。聚类分析表明,220个杂交玉米材料间的相似系数为0.62~0.95。以0.62为阈值可将220个杂交玉米材料划分为两大类群,第Ⅰ类群包括10个玉米材料,占4.5%;第Ⅱ类群包括210个玉米材料,占95.5%。本研究筛选的20对SSR引物多态性高、扩增效果好、稳定性好,可作为云南玉米杂交种特异性鉴定的核心引物。

Screening and Diversity Analysis of Core Primers on SSR Markers for Yunnan Maize Hybrids

In order to reduce the cost of primer synthesis, accelerate the process of identifying hybrid maize varieties, the core primers of the SSR markers which are suitable for the identification of Yunnan maize hybrids were screened.In this study, 40 pairs of SSR primers recommended in the SSR identification rules of maize were used. 20 pairs of primers with high polymorphism, effective and stable amplification were selected and recommended as the core primers for the specific identification of Maize Hybrids in Yunnan. Then,these primers were used for evaluating and analyzing the diversity of 220 hybrid maize materials in Yunnan Province. Totally 216 allelic variations were detected in 12 hybrid maize varieties, with an average of 10.80 alleles primers per pair. These primers could be used as core primers for Yunnan maize hybrids distinctness identification. 236 allele variations were detected within 20 SSR core primers for 220 maize hybrid materials, with an average of 11.80 alleles per pair, the average effective allele variations were 3.158 8, and the average Shannon-Weaver diversity information index was 3.028 2. Cluster analysis showed that the similarity coefficients of 220 maize hybrids were 0.62 to 0.95. With 0.62 as the threshold, 220 maize hybrid materials can be divided into two groups. Group Ⅰ included 10 maize materials with the proportion of 4.5%, other group includes 210 maize materials, the percentage was 95.5%. 20 pairs of SSR primer screened were high polymorphism, good amplification effect and good stability, they were recommended as core primers to specific identification distinctness of Yunnan maize hybrids.