| 香瓜根结线虫种类鉴定及杀线剂的室内毒力测定 |
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| 引用本文： | 李周容,孙燕芳,陈园,裴月令,冯推紫,龙海波.香瓜根结线虫种类鉴定及杀线剂的室内毒力测定[J].热带生物学报,2021,12(3):375-379. |
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| 作者姓名： | 李周容 孙燕芳 陈园 裴月令 冯推紫 龙海波 |
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| 作者单位： | 1.海南大学 植物保护学院，海口，570228 |
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| 基金项目： | 国家重点研发计划（2018YFD0201209）；中国热带农业科学院基本业务费专项（1630042017024）；农业农村部部门预算项目“粮经轮作模式关键技术优化提升与集成示范” |
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| 摘 要： | 利用形态学与分子生物学方法，对采集于海南省文昌市乌鸡塘村香瓜根系上的根结线虫种群进行鉴定，明确其为象耳豆根结线虫（Meloidogyne enterolobii），同时测定3种常用杀线剂对象耳豆根结线虫2龄幼虫的室内毒力。结果表明，95%阿维菌素原药对象耳豆根结线虫2龄幼虫的毒杀效果最好，LC50值为0.351 mg·L?1；85%噻唑膦原药和95%氟吡菌酰胺原药对象耳豆根结线虫2龄幼虫的LC50值分别为0.82 mg·L?1和7.783 mg·L?1。
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| 关 键 词： | 香瓜 根结线虫 鉴定 室内药剂筛选 |
| 收稿时间： | 2021-01-31 |
Identification and Laboratory Screening of Muskmelon Root-knot Nematodes |
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| Institution: | 1.College of Plant Protection, Hainan University, Haikou, Hainan 570228, China2.Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China |
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| Abstract: | A root-knot nematode population collected from Wujitang Village, Wenchang City, Hainan Province was identified by using morphological and molecular biological methods. The result showed that the root-knot nematode infesting the muskmelon was Meloidogyne enterolobii. Three commonly used nematicides were further tested to determine their toxicity to the second-instar larvae of M. enterolobii under laboratory conditions. The tests showed 95% avermectin had the best toxic effect on the 2nd instar larvae, with an LC50 value of 0.351 mg·L?1, followed by 85% fosthiazate and 95% fluopyram, with the LC50 values of 0.82 mg·L?1 and 7.783 mg·L?1, respectively. These results will provide reference for the precise control and prevention of M. enterolobii in the muskmelon field in Hainan. |
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