伊蚊3HKT基因RNAi载体构建及口服对伊蚊的致死作用

构建伊蚊3HKT基因的RNAi表达载体pMaa7IR/3HKTIR，转化莱茵衣藻，将得到的转基因藻株喂饲伊蚊幼虫。结果表明，3HKT RNAi转基因藻株对伊蚊有一定的致死作用。伊蚊是登革热、寨卡病毒病、黄热病、基孔肯雅病的媒介昆虫；微藻是蚊子幼虫天然食物，在自然界广泛存在，并且小球藻、衣藻等可大规模生产、生产成本低廉，因此，可长期将其大规模投放在封闭区域，形成优势藻种。本研究可为生物杀蚊，阻断登革热、寨卡病毒病等恶性传染病的流行提供新思路。

Construction of RNAi Vector of 3HKT Gene and Its Lethal Effect on Aedes aegypti

Mosquito-borne infectious diseases are seriously threatening the lives and health of humans all over the world. Among them, Aedes aegypti is a vector insect that spreads dengue fever, Zika virus disease, yellow fever, and chikungunya. Therefore, the control of Aedes aegypti plays an important role in the control and prevention of the aforementioned infectious diseases. In this context, an RNAi expression vector pMaa7IR/3HKTIR of the Aedes aegypti 3HKT gene was constructed and transformed to Chlamydomonas reinhardtii by the glass bead method. The transgenic algal strains were then used to feed Aedes larvae. The results showed the 3HKT RNAi transgenic algae was lethal to A. aegypti larvae. Microalgae are natural food for mosquito larvae and they are widespread in nature. Microalgae, such as Chlorella, Chlamydomonas, etc. can be produced at a large scale in the factory with low cost and placed into closed areas to form dominant algae species. This study provides a new idea for biological mosquito control to prevent the spread of dengue fever, zika virus disease, and other infectious diseases transmitted by mosquitos.