花生脂磷酸磷酸酶基因家族的克隆与表达分析

为探究脂磷酸磷酸酶(LPP)基因家族在花生中的功能,本研究从花生中克隆得到8个LPP基因,分别命名为AhLPP1、AhLPP2、AhLPP4、AhLPPβ1、AhLPPβ2、AhLPPγ、AhLPPδ、AhLPPε,分别编码335、322、284、228、198、227、403和293个氨基酸,均属于LPPs蛋白质家族。随后对克隆的基因进行序列相似性比对和系统发育分析;同时通过实时荧光定量PCR(RT-qPCR)检测AhLPP基因在花生不同组织、不同发育时期、5种激素和4类非生物逆境胁迫下的表达情况。结果显示,这些基因与其他植物的LPPs蛋白有较高的相似性,可能参与花生种子油脂的合成。在5种激素与4类非生物胁迫处理下AhLPP2、AhLPPγ、AhLPPε基因均显著诱导表达,其余LPP基因在部分处理下显著诱导表达。本研究为阐明LPP类基因在花生油脂合成和逆境胁迫抗性的功能奠定了理论基础,丰富了花生品种改良的基因资源。

Cloning and Expression Analysis of Lipid Phosphate Phosphatase (LPP) Gene Family in Peanut

To study the functions of lipid phosphate phosphatases in peanut, eight LPP genes were cloned in this study, which were named as AhLPP1, AhLPP2, AhLPP4, AhLPPβ1, AhLPPβ2, AhLPPγ, AhLPPδ, AhLPPε respectively. They encodes 335, 322, 284, 228, 198, 227, 403, 293 amino acids, respectively. All of them belong to the LPPs protein family. Subsequently, the cloned genes were used to perform sequence similarity comparison and phylogenetic analysis by related tools. At the same time, the expression of AhLPP genes in different tissues, different developmental stages of seeds and undr five kinds of hormones and four kinds of four kinds of abiotic stresses were delected by quantitative real-time PCR. The results showed that the AhLPP genes were highly similar to LPPs of other species and may participate in the synthesis of seed oils. AhLPP2, AhLPPγ, AhLPPε genes were differentially expressed under all kinds of treatment with five hormones and four abiotic stresses, while other LPP genes were differentially expressed following exposure to some kinds of treatments. This study provides a theoretical basis for clarifying the function of LPP genes in the synthesis of peanut oil and improving abiotic stress resistance, and enriches new gene resources for seed improvement of peanut.