初烤烟叶中烟碱和新植二烯的提取工艺研究

为探究云南烟油的成分以及烟碱和新植二烯的提取工艺,本试验以云南典型初烤烟叶为原料,通过超临界CO2萃取、超声辅助萃取、索式回流萃取和溶剂萃取4种方法提取初烤烟叶中烟碱和新植二烯,采用气相色谱-质谱(GC-MS)分析萃取物组成,比较不同萃取方法和萃取溶剂下,烟叶萃取物得率及所得萃取物中烟碱和新植二烯的相对含量.结果 表明...

Study on Extraction Technology of Nicotine and Neophytadiene From Flue-Cured Tobacco Leaves

In order to study the ingredients of Yunnan tobacco essential oil and the extraction tecnology of nicotine and neophytadiene, the typical flue-cured tobacco leaves in Yunnan were used as raw materials to extract nicotine and neophytadiene by CO₂ supercritical fliud extraction(CO₂-SFE), ultrasonic assisted extraction, Soxhlet reflux extraction and solvent extraction. The composition and content in the extracts were analyzed by gas chromatography-mass spectrometry (GC-MS). The extraction rates and the relative contents of nicotine and neophytadiene in the obtained extracts were compared with different extraction methods and solvents, and the suitable extraction methods for nicotine and neophytadiene in Yunnan flue-cured tobacco leaves were determined. The results showed that the yields of tobacco essential oil from high to low by the four processes were soxhlet extraction, solvent extraction, ultrasound-assisted extraction and CO₂-SFE with ethanol as extractant. Nicotine, neophytadiene, n-hexadecanoic acid (Z, Z), 9,12,15-octadecatrienoic acid, 1,5,9-trimethyl-12-(1-methyl ethyl) -4,8,13-cyclotetratriene-1,3-diol and vitamin E were the main components in all the extracts obtained under different extraction processes. The total relative contents of nicotine and neophytadiene were as follows: soxhlet extraction(75.58%, 2.85%) > CO₂-SFE(35.01%, 26.80%) > ultrasound-assisted extraction(32.28%, 28.24%) > solvent extraction(9.56%, 45.20%). The results of this study provide a theoretical basis for the study on the ingredients in Yunnan smoke oil, as well as nicotine and neophytadiene.