一株贝莱斯芽孢杆菌的分离与鉴定

为获得高温高效分解茶渣的菌株。利用高温特殊生境分离方法,从废弃茶渣中分离到有分解茶渣作用的5株菌,其中Fb菌株产生的蛋白酶、纤维素酶的活力均最高。经形态学、生理生化特性以及生长特性等研究,并克隆Fb菌株的16S rDNA基因序列和gyrB基因序列,进行系统发育学分析。结果表明,Fb属贝莱斯芽孢杆菌(Bacillus velezensis),可耐受55℃的高温。最适生长条件为：装液量30 mL、摇床转速分120 r/min、培养温度42~45℃、初始pH 5.0~7.0、培养时间16 h、盐度1.0%~6.0%。本研究首次在茶渣中分离筛选出贝莱斯芽抱杆菌,为茶渣开发研究提供理论依据。

A Strain of Bacillus velezensis: Isolation and Identification

In order to obtain strains which can decomposing tea residue in high-temperature and with high efficiency, we used the high temperature special habitat separation method, and isolated 5 bacteria strains that could decompose tea residues. Among them, the protease and cellulase produced by the Fb strain had the highest activity. After studying the morphology, physiological and biochemical characteristics and growth characteristics of the Fb strain, the 16S rDNA gene sequence and the gyrB gene sequence of the strain were cloned, and the phylogenetic analysis was carried out. The results showed that Fb belonged to Bacillus velezensis, which could tolerate the high temperature of 55℃. The optimal growth condition of the strain was: the filling volume of 30 mL, the rotation speed of the shaking table of 120 r/min, the incubation temperature of 42-45℃, the initial pH of 5.0-7.0, the incubation time of 16 h, and the salinity of 1.0%-6.0%. For the first time, Bacillus velezensis is isolated and screened out of tea residue, and the study could provide a theoretical basis for the development and research of tea residue.