伞形花内酯处理对采后徐香猕猴桃果实灰霉病抗的影响

为探讨伞形花内酯处理对猕猴桃果实采后灰霉病的控制效应,本试验研究了伞形花内酯对灰霉菌孢子和生长的影响,以及对美味猕猴桃徐香损伤接种灰霉菌后果实病害发展、抗病相关酶活性和抗性物质的影响。结果表明,离体条件下,0.5和1.0 mg·mL^-1伞形花内酯能够显著抑制灰霉菌孢子的萌发和生长;0.5 和1.0 mg·mL^-1伞形花内酯处理能够抑制损伤接种灰霉菌猕猴桃果实病斑的扩张,显著提高果肉几丁质酶(CHT)、β-1,3 葡聚糖酶(GLU)、苯丙氨酸解氨酶(PAL)、4-香豆酰辅酶A连接酶(4CL)、过氧化物酶(POD)和多酚氧化酶(PPO)的活性,提高果实贮藏后期总酚、类黄酮和木质素的含量,因而诱导了猕猴桃采后果实对灰霉病的抗性。本研究为应用外源伞形花内酯控制猕猴桃采后果实灰霉病害提供了理论依据。

Eeffect of Umbelliferone Treatment on Disease Resistance to Botrytis cinerea in Kiwifruit cv. Xuxiang During Storage

For investigating the control efficiency of umbelliferone application on postharvest Botrytis cinerea in kiwifruit, the effects of umbelliferone treatment on B. cinerea spores and growth, the activities of defense related enzymes and the resistance-related substances after inoculation with B. cinerea in kiwifruit (Actinidia deliciosa. cv. Xuxiang) were studied during storage at room temperature. The results showed that 0.5 and 1.0 mg·mL^-1umbelliferone effectively inhibited the spore germination and enlargement of colony diameter of B. cinerea in vitro. Also, the umbelliferone treatment with 0.5 and 1.0 mg·mL^-1concentration reduced the expansion of the lesion area in kiwifruit inoculated with B. cinerea, and effectively increased the activities of defense-related enzymes, including chitinase (CHT), β-1, 3-glucanase (GLU), phenylalanine ammonia lyase (PAL), 4-coumarate CoA ligase (4-CL), peroxidase (POD) and polyphenol oxidase (PPO) in flesh of the kiwifruit, along with the increases in contents of resistant substances in treated kiwifruit, such as total phenolics, flavonoids and lignin, and thereby, decreased the incidence of gray mold in kiwifruit during postharvest. Our present work might provide a theoretical basis for the control of gray mold in kiwifruit by application of umbelliferone during storage.