鳄鱼源气单胞菌的毒力基因与耐药特性分析

对海南地区患病暹罗鳄（Crocodylus siamensis）体内分离出的10株气单胞菌(5株嗜水气单胞菌、3株达卡气单胞菌、1株简达气单胞菌和1株维氏气单胞菌)进行了毒力基因、耐药基因的检测以及抗生素药物敏感性试验。结果表明:气溶素、肠毒素、蛋白酶等基因检出率较高，其中，lip和ela 2种毒力基因检出率高达100%；喹诺酮类、四环素类、磺胺类、β?内酰胺类、氨基糖苷类耐药基因检出率大于50%；气单胞菌对喹诺酮类抗生素、四环素类抗生素、氨基糖苷类抗生素、氯霉素类和碳青霉烯类抗生素的敏感率较高，而对于β?内酰胺类抗生素、磺胺类抗生素和糖肽类抗生素耐药率较高。

Characteristics of Virulence Genes and Antibiotic Resistance of Aeromonas spp. Isolated from Siamese crocodile

A total of 10 strains of Aeromonas spp. were isolated from sick Siamese crocodile in Hainan, including 5 strains of Aeromonas hydrophila, 3 strains of Aeromonas dhakensis, 1 strain of Aeromonas veronii and 1 strain of Aeromonas jandaei, and tested for virulence genes, antibiotic resistance genes, and antibiotic susceptibility. The results showed that aerosol genes, enterotoxin genes and protease genes had a higher detection rate, of which two virulence genes, lip and ela, had a detection rate of up to 100% and the genes resistant to quinolones, tetracyclines, sulfonamides, β-lactams, and aminoglycoside had a detection rate of higher than 50%. Antibiotic susceptibility testing showed that Aeromonas spp.were more sensitive to quinolone, tetracycline, aminoglycoside, chloramphenicol and carbapenem antibiotics, and more resistant to β-lactam, sulfonamides and glycopeptide antibiotics.