拮抗芽孢杆菌对蒙古黄芪主要药效成分的影响

探讨黄芪根腐病拮抗芽孢杆菌SXKF16-1(Bacillus atrophaeu)和SXKF16-2(B. methylotrophicus)对黄芪主要药效成分的影响,旨在为后续多功能制剂的开发提供依据。采用灌根法对健康黄芪苗浇注拮抗菌发酵液,HPLC-UV-ELSD法对10种药效成分进行测定。结果表明,毛蕊异黄酮葡萄糖苷、芒柄花苷、紫檀烷苷、异黄烷苷、毛蕊异黄酮、芒柄花素和黄芪皂苷Ⅳ、Ⅲ、Ⅱ、Ⅰ在各自标准曲线相应的线性范围内线性关系良好(r≥0.9950),试验测定结果可靠。拮抗芽孢杆菌SXKF16-1和SXKF16-2处理对药效成分毛蕊异黄酮葡萄糖苷、芒柄花苷、紫檀烷苷、异黄烷苷、毛蕊异黄酮、芒柄花素、黄芪皂苷Ⅱ、黄芪皂苷Ⅳ的含量均无明显影响,但显著促进黄芪皂苷Ⅰ和黄芪皂苷Ⅲ含量的升高。2组处理与对照相比,总黄酮含量均无明显变化,总皂苷含量显著升高。拮抗芽孢杆菌SXKF16-1和SXKF16-2对黄芪主要药效成分的含量无负面影响,可促进某些皂苷类成分的积累,具备开发为多功能制剂的潜力。

Effects of Antagonistic Bacillus on the Main Active Components of Astragalus membranaceus var. mongholicus

The effects of antagonistic Bacillus SXKF16-1 (Bacillus atrophaeu) and SXKF16-2 (B. methylophicus) on the main active components of Astragalus membranaceus var. mongholicus were investigated so as to provide a basis for the development of multi-functional preparations. Healthy A. membranaceus var. mongholicus seedlings were root-irrigated with the fermentation of antagonistic Bacillus, and ten effective components were determined by HPLC-UV-ELSD. There was a good linear relationship (r≥0.9950) in the corresponding linear range of the respective standard curve of calycosin-7-O-β-D-glucoside, ononin, (6aR,11aR)-9,10-dimethoxyptercarpan, 8,2'-dihydroxy-3,4-dimethoxyisoflavan-7-O-β-D-glucopyranoside, calycosin, formononetin, astragaloside Ⅳ, Ⅲ, Ⅱ, Ⅰ, and thus the test results were reliable. Treated with antagonistic Bacillus SXKF16-1 and SXKF16-2, the contents of such A. membranaceus var. mongholicus active components as calycosin-7-O-β-D-glucoside, ononin, (6aR,11aR)-9,10-dimethoxyptercarpan, 8,2'-dihydroxy-3,4-dimethoxyisoflavan-7-O-β-D-glucopyranoside, calycosin, formononetin, astragaloside Ⅱ and Ⅳ showed no significant change, but the contents of astragaloside Ⅰ and Ⅲ increased significantly. Compared with the control group, the content of total flavonoids in the two treatment groups had no significant change, while the content of total saponins increased significantly. Antagonistic Bacillus SXKF16-1 and SXKF16-2 have no negative effect on the content of main active components of A. membranaceus var. mongholicus, and can promote the accumulation of some saponins; and therefore have the potential of being developed into multi-functional preparations.