黎药光叶巴豆枝叶的化学成分分析

为研究光叶巴豆枝叶的化学成分，笔者采用硅胶柱色谱、Sephadex LH-20、半制备高效液相色谱等多种色谱技术进行化合物的分离纯化，通过化合物的波谱数据和理化性质鉴定其结构。从光叶巴豆枝叶95%乙醇提取物中分离得到12个化合物，分别鉴定为:(3S，5R，6S，7E，9R)-3，6-dihydroxy-5，6-dihydro-β-ionol ( 1 )、吐叶醇( 2 )、4，5-dihydroblumenol A ( 3 )、5-epi-eudesma-4(15)-ene-1β，6β-diol ( 4 )、aromadendrane-4β，10β-diol ( 5 )、ent-4(15)-eudesmene-1β，6α-diol ( 6 )、(7R*)-opposit-4(15)-ene-1β，7-diol ( 7 )、丁香脂素( 8 )、rel-(3R，3′S，4R，4′S)-3，3′，4，4′-tetrahydro-6，6′-dimethoxy3，3′-bi-2H-benzopyran]-4，4′-diol ( 9 )、(+)?皮树脂醇( 10 )、thero-ficusesquilignan A ( 11 )和反式对羟基桂皮酸甲酯( 12 )，这些化合物均为首次从光叶巴豆中分离得到。采用贝尔曼漏斗法和MTT法对化合物 1 ～ 12 进行了生物活性测试，结果表明，化合物 8 ～ 12 对全齿复活线虫具有较好的致死活性，且所有化合物均无细胞毒活性。

Chemical Constituents from the Twigs and Leaves of Croton Laevigatus, a Li Folk Medicine

The twigs and leaves of Croton laevigatus, a Li Folk Medicine, were extracted with ethanol, from which compounds were separated and purified by using column chromatography on silica gel, sephadex LH-20 and semi-preparative HPLC, and their structures were identified on the basis of spectroscopic data and physicochemical properties. Twelve compounds were separated from the 95% ethanol extract of the twigs and leaves of C. laevigatus, including (3S, 5R, 6S, 7E, 9R)-3,6-dihydroxy-5,6-dihydro-β-ionol (1), vomifoliol (2), 4,5-dihydroblumenol A (3), 5-epi-eudesma-4(15)-ene-1β, 6β-diol (4), aromadendrane-4β, 10β-diol (5), ent-4(15)-eudesmene-1β, 6α-diol (6), (7R*)-opposit-4(15)-ene-1β, 7-diol (7), (+)?syringaresinol (8), rel-(3R, 3′S, 4R, 4′S)-3,3′,4,4′-tetrahydro-6, 6′-dimethoxy3,3′-bi-2H-benzopyran]-4,4′-diol (9), (+)?medioresinol (10), thero-ficusesquilignan A (11), trans-p-hydroxycinnamic acid methyl ester (12). Compounds 1?12 were obtained from C. laevigatus for the first time. The bioassay of the compounds 1?12 was conducted with Behrman and MTT methods. The bioassay showed that the compounds 8?12 had significant nematicidal activity against Panagrellus redivivus, and that all the compounds were inactive against the selected human cancer cell lines.