大豆黄酮对奥利亚罗非鱼生长及相关激素水平、血液生化指标的影响

挑选体质量相近的奥利亚罗非鱼，随机放入网箱，分为对照组（基础日粮）、大豆黄酮（Da）组（基础日粮＋Da10mg／kg），并各设2个重复，试验期为32d。结果，与对照组比较，Da组雄鱼日增重提高14．9％（P〈0．05），体质量特定生长率增加15．2％（P〈0．05），生长激素（GH）水平提高29．1％，甲状腺激素（T3）水平提高13．2％，睾酮水平提高27．3％，雌二醇水平下降22．8％；雌鱼日增重及体质量特定生长率变化不大（P〉0．05），GH水平提高24．8％。T3、睾酮水平几无变化，雌二醇水平下降10．5％。Da组雄、雌鱼脲氨水平分别下降51．5％、17．3％（P〈0．05）；血清抗氧化能力分别上升79．5％、48．1％（P〈0．05）；肝抗氧化能力分别上升73．9％、33．3％（P〈0．05）；血钙、血糖水平变化不大。结论：Da可能通过提高生长激素和睾酮水平促进奥利亚罗非鱼雄鱼生长。

Effects of Daidzein on Growth, Hormone and Physio-biochemical Parameters Levels in Oreochromis aureus

Daidzein(Da) is isoflavonic phytoestrogens,which is known to be involved in the E_2 levels.In the present study,Da was used to evaluate its effect on the growth,hormone and physio-biochemical parameters levels in Oreochromis aureus.Da(10 mg/kg) was added into the diet for 32 days to 23 male and 7 female oreochromis aureus with similar weight.Compared with control,the treatment promoted significantly the growth of male fish.To which,average daily body weight gain(DBG) increased 14.9%(P<0.05),specific growth rate(SPR) increased 15.2%(P<0.05),the serum GH,T_3 and testosterone levels of male elevated 29.1%,13.2% and 27.3%,E_2 decreased 10.5%;T_3 and testosterone kept unchanged.In serum physio-biochemical parameters,the SUN level in male and female were decreased 51.5%,17.3%(P<(0.05),) respectively,the serum antioxidant capacity significantly increased 79.5%,48.1%(P<0.05),the serum antioxidant capacity significantly increased(73.9%,)33.3%(P<0.05),respectived,the levels of serum calcium,serum glucose were unchanged(P>0.05).These results indicated that Da could increase serum GH and testosterone levels and promote growth in male Oreochromis aureus.