凤尾蕨科植物蜈蚣草的组织培养

[目的]对凤尾蕨科植物蜈蚣草的组织培养方法进行研究。[方法]以野外采集的叶芽为外植体,以1/2 MS＋3%蔗糖＋0.7%琼脂为基本培养基,通过激素配比试验,筛选蜈蚣草愈伤组织诱导和继代培养的培养基配方。[结果]从外植体诱导出愈伤组织最佳培养基配方为1/2 MS＋3%蔗糖＋0.7%琼脂＋0.5 g/L PVP＋0.1 mg/L KT＋0.5 mg/L 2,4-D;从愈伤组织诱导出GGB和从GGB诱导出幼苗最佳培养基配方为1/2MS＋3%蔗糖＋0.7%琼脂＋0.5 g/L PVP＋1.0 mg/L KT＋0.01 mg/L 2,4-D;另外,使用1/2 MS＋3%蔗糖＋0.7%琼脂＋0.5 g/L PVP＋0.5 mg/L 2,4-D做继代培养可以使愈伤组织持续增殖。[结论]研究直接使用野外材料组织培养,简化了试验步骤,是一种方便快速的蜈蚣草组培方法。

Research on Tissue Culture of Pteris vittata L.

[Objective] The aim was to study the tissue culture of Pteris vittata L.which was a species of fern belonging to the genus Pteris.[Method] The collected leaf bud was used as explants,and the 1/2 MS+3% sucrose+0.7% agar was used as the basic medium,the phytohormone ratio testing was used to screen the medium formula for callus induction and subculture of P.vittata.[Result] The best medium formula for the callus induced from explants was: 1/2 MS+3% sucrose+0.7% agar+0.5 g/L PVP+0.1 mg/L KT+0.5 mg/L 2,4-D;the best medium formula for the GGB induced from callus and the seedlings induced from GGB was: 1/2MS+3% sucrose+0.7% agar+0.5 g/L PVP+1.0 mg/L KT+0.01 mg/L 2,4-D;in addition,the use of 1/2 MS+3% sucrose+0.7% agar+0.5 g/L PVP+0.5 mg/L 2,4-D for the subculture could make the continued proliferation of callus.[Conclusion] The direct use of field material for tissue culture had simplified the test procedure,thus being a rapid tissue culture method for P.vittata.