丙酮沉淀法提取金花茶多糖的研究

目的]确定丙酮沉淀法提取花金花茶（Camellia chrysantha（Hu）Tuyama）多糖的最佳工艺条件。方法]以金花茶为研究对象,以水为溶剂,在一定的条件下提取金花茶多糖,提取液用丙酮进行沉淀,选取沉淀剂用量、沉淀时间和离心时间3个因素做单因素试验,然后以离心时间、丙酮加入量、沉淀时间作为因素,进行正交试验,来确定最佳提取条件。结果]丙酮沉淀法的最佳工艺条件为：离心时间为15 min,丙酮加入量为20 ml、沉淀时间为12 h,在此条件下的所得粗茶多糖含量量为0.003 7 g。结论]该研究建立的检测方法简便、快速,具有较强的实用性。

Study on the Extraction of Polysaccharides from Camellia chrysantha (Hu) Tuyama by Acetone Precipitation

Objective] To determine the optimum extraction conditions of polysaccharides from Camellia chrysantha(Hu) Tuyama by acetone deposition method.Method] Extracts of polysaccharides that were obtained from Camellia chrysantha(Hu) Tuyama as research object and water as solvent under certain conditions were precipitated with acetone.Using the amount of acetone,precipitation time and centrifugation time as investigated factors,single factor and orthogonal test were carried out to optimize the extraction process of polysaccharides from Camellia chrysantha(Hu) Tuyama.Result] The optimum extraction conditions of polysaccharides from Camellia chrysantha(Hu) Tuyama by acetone deposition method were obtained: centrifugation time of 15 min,acetone amount of 20 ml and precipitation time of 12 h.Under the above condition,the weight of polysaccharides was 0.003 7 g.Conclusion] The method established in this study was simple,quick and practical.