2种中药提取物对番茄灰霉病菌和番茄早疫病菌的抑制活性研究

目的]研究白术和白头翁2种中药提取物的杀菌活性,为其在植物源农药上的开发利用奠定基础。方法]采用菌丝生长速率法,研究了白术和白头翁2种植物提取物对番茄灰霉病菌和番茄早疫病菌的抗菌活性。结果]供试2种中药提取物对番茄灰霉病菌和番茄早疫病菌均具有较好的抗菌活性,其中白头翁提取物的抗菌活性最好,在供试提取物质量吸度为10 mg/ml时,72 h白头翁正丁醇提取物对番茄灰霉病菌的抑制率达80.25%,白术石油醚提取物对番茄灰霉病菌的抗菌活性较好,抑制率为71.36%,且提取物的抗菌活性随着吸度的增大而增强,白头翁正丁醇提取物对番茄灰霉病菌的EC50为2.93 mg/ml,白术石油醚提取物对番茄灰霉病菌的EC50为5.31 mg/ml。结论]白头翁提取物对番茄灰霉病菌和番茄早疫病菌的抑制活性较好,值得进一步研究。

Antifungal Activity of Botanical Extracts against Botrytis cinerea and Alternaria Solani

Objective] The antifungal activity of the extracts from Atractylodes macracephal Koidz and Pulsatilla chinensis Bunge Regel,against Botrytis cinerea and Alternaria solani were studied under the condition of laboratory,in order to develop and utilize these two plants.Method] The mycelium growth rate test was applied to measure the antifungal activities of extracts against fungi.Result] The extracts of all the two plants showed strong antifungal activity against the target pathogenic fungi,especially the antifungal activity of the extract from Pulsatilla chinensis Bunge Regel was stronger and more stable.The inhibition rate to the mycelium growth of Botrytis cinerea was 80.25% and the EC50 was 2.93 mg/ml.At the same concentration,the extract from Atractylodes macracephal Koidz showed little inhibition to Botrytis cinerea and Alternaria solani,the inhibition rate to the mycelium growth of Botrytis cinerea was 71.36% and the EC50 was 5.31 mg/ml.Conclusion] The extracts from Pulsatilla chinensis Bunge Regel showed the stronger antifungal activity against Botrytis cinerea and Alternaria solani.