| 黑皮果蔗花叶病茎尖脱毒技术研究 |
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| 引用本文： | 唐红琴,淡明,李松,余坤兴,黄诚华,刘丽敏,刘红坚,卢曼曼.黑皮果蔗花叶病茎尖脱毒技术研究[J].安徽农业科学,2011,39(25):15345-15346. |
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| 作者姓名： | 唐红琴 淡明 李松 余坤兴 黄诚华 刘丽敏 刘红坚 卢曼曼 |
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| 作者单位： | 广西壮族自治区甘蔗研究所,广西南宁,530007;广西壮族自治区甘蔗研究所,广西南宁,530007;广西壮族自治区甘蔗研究所,广西南宁,530007;广西壮族自治区甘蔗研究所,广西南宁,530007;广西壮族自治区甘蔗研究所,广西南宁,530007;广西壮族自治区甘蔗研究所,广西南宁,530007;广西壮族自治区甘蔗研究所,广西南宁,530007;广西壮族自治区甘蔗研究所,广西南宁,530007 |
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| 基金项目： | 广西甘蔗研究所基本科研业务专项，现代农业产业技术体系建设专项 |
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| 摘 要： | 目的]利用果蔗热带种（Badila）茎尖培养进行甘蔗花叶病的脱毒研究。方法]采用黑皮果蔗茎尖作为外植体进行了组织培养,并在生根阶段和假植阶段进行了甘蔗花叶病毒的分子检测。结果]初代培养基为MS＋0.50 mg/L6-BA＋0.01 mg/LNAA＋30 g/L蔗糖,继代培养基为MS＋0.20 mg/L6-BA＋0.01 mg/LNAA＋30 g/L蔗糖。甘蔗花叶病毒检测结果为阴性。结论]利用热处理结合茎尖培养能够有效脱除花叶病毒。
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| 关 键 词： | 黑皮果蔗 甘蔗花叶病:茎尖培养 |
Shoot-tip Virus-free Techniques of Black Sugarcane Mosaic Disease |
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| Institution: | TANG Hong-qin et al(Guangxi Sugarcane Research Institute,Nanning,Guangxi 530007) |
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| Abstract: | Objective] The detoxification of sugarcane mosaic disease was studied by mean of sugarcane tropical species(Badila) shoot-tip culture.Method] The shoot tip of black sugarcane was used as explants for tissue culture,and the sugarcane mosaic virus in rooting phase and single plant planting stage was detected at molecular level.Result] The early generations medium was MS+0.05 mg/L 6-BA+0.01 mg/L NAA+30 g/L sucrose,and the subculture medium was MS+0.20 mg/L BA+0.01 mg/L NAA+30 g/L sucrose.The molecular detection of sugarcane mosaic virus in rooting phase and single plant planting stage showed negative.Conclusion] Shoot-tip culture combined with the use of heat treatment can be effective removal mosaic virus. |
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| Keywords: | Black sugarcane Mosaic disease Shoot-tip culture |
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