酿酒酵母抗氧化酶系统对NaCl和Na_2CO_3胁迫的生理响应

目的]研究酿酒酵母SOD和CAT对NaCl和Na2CO3胁迫的生理响应。方法]采用分光光度法,测定不同浓度NaCl和Na2CO3胁迫条件下酵母菌SOD和CAT的活性指标;采用比浊法,测定不同浓度NaCl和Na2CO3胁迫对酵母铜/锌超氧化物歧化酶缺失菌株（sod1Δ）和过氧化物酶缺失菌株（ctt1Δ）生长的影响。结果]随着NaCl和Na2CO3胁迫浓度的升高,酵母细胞内SOD和CAT活性呈现上升趋势;与野生型菌株相比,NaCl和Na2CO3胁迫对sod1Δ和ctt1Δ基因缺失菌株产生更加明显的生长抑制作用。结论]SOD和CAT在酿酒酵母抵抗NaCl和Na2CO3胁迫中发挥重要作用。

Physiological Response of Antioxidant Enzyme System to NaCl and Na2CO3 Stress in Saccharomyces cerevisiae

Objective] The research aimed to investigate the physiological response of superoxide dismutase and catalase to NaCl and Na2 CO3 stress in Saccharomyces cerevisiae.Method] Under different concentration of NaCl and Na2 CO3 stress condition,the activities of superoxide dismutase and catalase were measured by the means of spectrophotometry.The effects of different concentration of NaCl and Na2 CO3 stress on the growth of sod1Δ and ctt1Δ mutants were tested by turbidimetry.Results]With the increase of NaCl and Na2 CO3 concentrations,the activities of the superoxide dismutase and catalase activity increased.Compared with the wild type yeast strain,NaCl and Na2 CO3 stress caused more growth-inhibitory effects to sod1Δ and ctt1Δ mutants.Conclusion] The superoxide dismutase and catalase were essential for the tolerance to NaCl and Na2 CO3 stress in Saccharomyces cerevisiae.