| 转反义PLDγ基因小麦的品质性状分析 |
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| 引用本文： | 王瑞霞,钱兆国,丛新军,牟秋焕,米勇,孙宪印,王超.转反义PLDγ基因小麦的品质性状分析[J].山东农业科学,2011(7):15-17,20. |
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| 作者姓名： | 王瑞霞 钱兆国 丛新军 牟秋焕 米勇 孙宪印 王超 |
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| 作者单位： | 泰安市农业科学研究院,山东泰安,271000 |
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| 基金项目： | 现代农业产业技术体系项目(nycytx-03)资助 |
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| 摘 要： | 利用穗茎注射法将反义PLDγ基因导入普通小麦兰考906，对经特异PCR扩增和PCR—Southern杂交技术筛选出的转基因植株及其受体进行了品质性状分析。麦谷蛋白电泳结果显示，转基因株系的HMW—GS亚基组成发生了变化，转基因株系缺少了受体的10亚基，新出现了受体所没有的8和12亚基。醇溶蛋白电泳结果显示，转基因株系与受体相比存在显著差异，主要体现在转基因后代中出现了新带或者缺少了受体的部分谱带及表达量的差异。转基因株系的品质性状有所改善，其幅度大小因转化株系的不同而异。
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| 关 键 词： | 转基因小麦 品质分析 差异 |
Analysis of Quality Characters for Transgenic Wheat with Anti-PLDγ Gene |
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| WANG Rui-xia,QIAN Zhao-guo,CONG Xin-jun,MU Qiu-huan,MI Yong,SUN Xian-yin,WANG Chao.Analysis of Quality Characters for Transgenic Wheat with Anti-PLDγ Gene[J].Shandong Agricultural Sciences,2011(7):15-17,20. |
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| Authors: | WANG Rui-xia QIAN Zhao-guo CONG Xin-jun MU Qiu-huan MI Yong SUN Xian-yin WANG Chao |
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| Institution: | WANG Rui-xia,QIAN Zhao-guo,CONG Xin-jun,MU Qiu-huan,MI Yong,SUN Xian-yin,WANG Chao(Taian Academy of Agricultural Sciences,Taian 271000,China) |
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| Abstract: | The antisense phospholipase Dγ(PLDγ) gene was transfered into the wheat variety LK906 by earstem injecting method.The quality characters were analyzed for transgenic plants by PCR and PCR-Southern techniques.The results of HMW-GS electrophoresis showed that the subunit composition of transgenic lines was altered.The receptor’s subunit 10 disappeared and the subunits 8 and 12 appeared in transgenic lines.The results of seed gliadin electrophoresis showed that there were significant differences between transgenic lines and receptor in the appearance of new bands and disappearance of the receptor’s,and the degree of expression was also different.All the quality characters were improved somewhat but the degree was different among different transgenic lines. |
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| Keywords: | Transgenic wheat Quality analysis Difference |
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