| 玉米种子纯度鉴定中DNA提取方法的比较研究 |
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| 引用本文： | 闫米格,许晶,骈跃斌,原佳敏,李素玲,张君捷.玉米种子纯度鉴定中DNA提取方法的比较研究[J].山东农业科学,2011(9):7-8,14. |
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| 作者姓名： | 闫米格 许晶 骈跃斌 原佳敏 李素玲 张君捷 |
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| 作者单位： | 山西省农业科学院食用菌研究所,山西太原,030031 |
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| 基金项目： | 山西省农业科学院攻关项目(YYZJC1013) |
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| 摘 要： | 分别以玉米干种子胚、幼芽和幼苗为材料提取DNA，并对所得DNA的纯度和质量进行检测，结果显示，利用玉米干种子胚提取的DNA质量及分子量大小与幼苗法相当，完全可以满足SSR分子标记对玉米杂交种纯度的鉴定。
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| 关 键 词： | 玉米杂交种 DNA提取 纯度 SSR分子标记 |
Comparison of DNA Extraction Methods for Purity Identification of Maize Seeds |
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| YAN Mi-ge,XU Jing,PIAN Yue-bin,YUAN Jia-min,LI Su-ling,ZHANG Jun-jie.Comparison of DNA Extraction Methods for Purity Identification of Maize Seeds[J].Shandong Agricultural Sciences,2011(9):7-8,14. |
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| Authors: | YAN Mi-ge XU Jing PIAN Yue-bin YUAN Jia-min LI Su-ling ZHANG Jun-jie |
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| Institution: | YAN Mi-ge,XU Jing,PIAN Yue-bin,YUAN Jia-min,LI Su-ling,ZHANG Jun-jie(Edible Fungi Research Institute,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China) |
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| Abstract: | In this study,the embryo of dry seeds,young buds and seedlings of maize were used as materials respectively to extract DNA,and the purity and quality of obtained DNA were detected.The results showed that the quality and molecular weight of DNA extracted with dry seed embryos were equivalent to those of DNA extracted with seedlings,and it could meet the need of SSR analysis and identification of maize seed purity. |
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| Keywords: | Maize hybrid DNA extraction Purity SSR molecular marker |
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