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| 抗生素在花生组织培养中的抑菌效应研究 | |
| 引用本文： | 任艳,王辉,石延茂,李双铃,李雷,崔潇,袁美.抗生素在花生组织培养中的抑菌效应研究[J].山东农业科学,2011(10):74-75,81. |
| 作者姓名： | 任艳 王辉 石延茂 李双铃 李雷 崔潇 袁美 |
| 作者单位： | 1. 山东省花生研究所/国家花生工程技术研究中心,山东青岛266100;中国海洋大学,山东青岛266100 2. 山东省花生研究所/国家花生工程技术研究中心,山东青岛,266100 3. 青岛市农业科学研究院,山东青岛,266100 |
| 基金项目： | 山东省农业科学院博士基金项目(2006YBS021); 青年基金项目(2005YQ010); 科技部“863”计划项目(2006AA100106) |
| 摘 要： | 为解决花生组织培养中细菌污染问题，在培养基中添加不同抗生素，在光照16h／d、光强3000lx、温度28℃的条件下培养，并观察材料受污染与生长情况。结果表明，培养基中添加抗生素能有效抑制花生组培苗的细菌性侵染，其中羧卞青霉素的抑茵效果最好；但添加抗生素对花生组培苗的生长有负面影响。 |
| 关 键 词： | 花生 组织培养 抗生素 |
Study on Bacteriostatic Efficacy of Antibiotic in Tissue Culture of Peanut |
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| REN Yan,WANG Hui,SHI Yan-mao,LI Shuang-ling,LI Lei,CUI Xiao,YUAN Mei.Study on Bacteriostatic Efficacy of Antibiotic in Tissue Culture of Peanut[J].Shandong Agricultural Sciences,2011(10):74-75,81. | |
| Authors: | REN Yan WANG Hui SHI Yan-mao LI Shuang-ling LI Lei CUI Xiao YUAN Mei |
| Institution: | REN Yan1,2,WANG Hui1,SHI Yan-mao1,LI Shuang-ling1,LI Lei3,CUI Xiao3,YUAN Mei1(1.Shandong Peanut Research Institute/ State Research Center of Peanut Engineering and Technology,Qingdao 266100,China,2.Ocean University of China,3.Qingdao Institute of Agricultural Sciences,China) |
| Abstract: | In order to solve the problem of bacterial pollution during peanut tissue culture,different antibiotics were added into the culture media.Under the culture conditions of 12 hours of light time every day,3 000 lx of light intensity and 28℃,the contaminated and growth situations of peanut culture seedlings were observed.The results showed that the addition of antibiotics could effectively restrain bacterial infection,in which,carbenicillin had the best control effect.But adding antibiotics had negative influence on growth of peanut seedlings. |
| Keywords: | Peanut Tissue culture Antibiotics |
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