过氧化氢及硫酸亚铁诱导洋葱表皮细胞凋亡

目的]探讨过氧化氢与硫酸亚铁对洋葱表皮细胞凋亡的影响。方法]分别设置4组处理：处理①：2 ml蒸馏水（对照组）;处理②：1 ml蒸馏水＋1 ml 4 mmol/LFeSO4;处理③：1 ml蒸馏水＋1 ml 2 mmol/LH2O2;处理④：1 ml 2 mmol/LH2O2＋1 ml 4 mmol/LFeSO4,处理洋葱表皮细胞24 h后,观察其凋亡情况。结果]处理①的细胞正常;处理②的细胞与对照组相似;处理③的细胞处于明显凋亡状态;处理④的细胞未出现明显凋亡。结论]FeSO4对洋葱表皮细胞凋亡有抑制作用。

Study on Apoptosis of Onion Epidermal Cells Induced by Hydrogen Peroxide and Ferrous Sulfate

Objective] The study aimed to investigate the effect of apoptosis of onion epidermal cells induced by hydrogen peroxide and ferrous sulfate.Method] The divided into four groups with ① 2 ml distilled water(control group),② 1 ml distilled water + 1 ml 2 mmol/L hydrogen Peroxide,③ 1 ml distilled water +1 ml 4 mmol/L ferrous sulfate,④1 ml 2 mmol/L hydrogen Peroxide +1 ml 4 mmol/L ferrous sulfate.Result] The cell of treatment ① were normal,cell of treatment ② were similar to control group,cell of treatment ③ were in obviously apoptotic status,cell of treatment ④ were not found phenomenon of apoptosis.Conclusion] Ferrous sulfate can inhibit the apoptosis of onion epidermal cells.